Profile of H3K27 epigenetic marks in porcine embryos during in vitro development
Notice bibliographique
Résumé
Changes in epigenetic marks on the lysine 27 of histone H3 (H3K27) appear to play a key role in lineage determination of mammalian embryos. Genes associated to organogenesis, morphogenesis and embryonic development are temporarily suppressed by trimethylation of H3K27 (H3K27me3) in pluripotent cells. However, the complete profile of epigenetic marks in the H3K27 during early stages of porcine embryo development is not entirely known. The aim of this study was to evaluate the acetylation (H3K27 ac) and methylation (mono-, di- and trimethyl; H3K27 me1, me2 and me3) profile of H3K27 during early development of in vitro-produced (IVP) porcine embryos. Oocytes/embryos (approximately 6 for each mark and developmental stages) were fixed at the following developmental stages: germinal vesicle oocytes (GV), metaphase II oocytes (MII), embryos at pronuclear stage (PN), two cells (2C), four cells (4C), eight cells (8C), D6 blastocysts (D6), and D8 blastocysts (D8). Oocytes/embryos were subjected to an immunofluorescence protocol using rabbit anti-H3K27 ac and anti-H3K27 me1, me2 and me3 primary antibodies (1:1000), and goat anti-rabbit Alexa Flour 488 as a secondary antibody (1:1000). Nuclei were stained with DAPI (10 µg/mL). The fluorescent signal for H3K27 ac was intensive in all developmental stages. H3K27 me1 signal was strong in GV, MII and in only one pronucleus, but the fluorescent signal was weak in 2C, 4C and 8C stage embryos. In D6 and D8 embryos, the H3K27 me1 signal became strong again. The signal for the H3K27 me2 mark was strong in GV and MII stage oocytes, but absent in PN. In embryos at 2C, 4C and 8C stages, the H3K27 me2 signal was very weak or absent, but it was detected again in D6 and D8 embryos. H3K27 me3 was intensely detected in GV, MII and in only one pronucleus. In 2C, 4C, 8C and D6 embryos, the H3K27 me3 signal was very weak or absent. Additionally, 29 D8 blastocysts were subjected to immunofluorescence for detection of H3K27 me3. Of these, 7 did not present any sign of fluorescence, 19 had some or all cells marked and 3 showed H3K27 me3 only in the inner cell mass. The results of this study indicate that the epigenetic marks on the H3K27 are highly dynamic during porcine embryogenesis, which suggests that H3K27 plays an important role in the regulation of gene expression during early embryo development.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».