P-029 Mesenchymal stem cells inhibit saccular aneurysm pathogenesis in a rabbit model
Notice bibliographique
Résumé
Introduction Intracranial aneurysms are relatively common entities, with rupture being a largely unpredictable event associated with significant morbidity and mortality. While current treatment modalities are effective at addressing mature aneurysms, disrupting aneurysm formation would potentially avoid the need for more invasive treatments. To inhibit saccular aneurysm pathogenesis would likely require blunting of the complex inflammatory cascade in the arterial wall. Mesenchymal stem cells (MSCs) are readily available cells found in adult tissues, such as bone marrow and adipose tissue, that have immunomodulatory properties and are immune evasive.1 They have been studied as potential treatments for many diseases associated with inflammation and have shown promise for clinical translation.2 While some studies have investigated MSCs as a treatment adjunct for aneurysms, none have examined their potential role in inhibiting aneurysm pathogenesis. We hypothesize that injecting MSCs IV will minimize the radiographic formation of aneurysms in a rabbit elastase-induced saccular aneurysm model by blunting the inflammatory process. Materials and methods Thirteen New Zealand White Rabbits were randomly assigned to either control (n=5) or intervention (n=8) groups. All rabbits underwent right common carotid artery saccular aneurysm creation using approximately 35 units of elastase as previously described in this accepted and effective model.(3) At both the time of surgery and 14 days post-operatively, intervention group rabbits received an IV injection of 5 × 106 MSCs in 2 mL saline, while control group rabbits received 2 mL of vehicle. On day 28, the right femoral artery was cannulated in all rabbits and digital subtraction angiography performed. Aneurysm volume was calculated and compared between groups. Results All rabbits tolerated the surgical procedures well with no peri-operative complications, and every procedure resulted in aneurysm formation. Mean volume of right common carotid saccular aneurysms in the control and intervention groups were 36.9 mm3 and 15.4 mm3, respectively. A Mann-Whitney U test was performed due to the heterogeneity of variances and found that the intervention group aneurysms were significantly smaller than control aneurysms (p=0.030). Conclusion This is the first study investigating the ability of MSCs to inhibit the formation of saccular aneurysms. We postulate that the observed effects are secondary to MSC-induced inhibition of the initial inflammatory cascade of aneurysm pathogenesis. MSCs are known to have immunomodulatory effects in many other scenarios, either by migrating to a site of inflammation or by releasing factors from afar.4 It is unclear by which mechanism the observed effect is occurring, but these findings suggest that MSCs may perhaps be utilized for inhibiting aneurysm formation. References . Kean TH, Lin P, Caplan AI, Dennis JE. MSCs: Delivery routes and engraftment, cell-targeting strategies, and immune modulation. Stem Cells Int. 2013. 2013: Article ID732742. . Kim N, Cho SG. Clinical applications of mesenchymal stem cells. Korean J Intern Med2013;28:387–402. . Hoh BL, Rabinov JD, Pryor JC, Ogilvy CS. A modified technique for using elastase to create saccular aneurysms in animals that histologically and hemodynamically resemble aneurysms in human. Acta Neurochir2004;146:705–711. . English K. Mechanisms of mesenchymal stromal cell immunomodulation. Immunol Cell Biol2013;91:19–26. Disclosures M. Avery: None. A. Sen: None. A. Mitha: None.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,001 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,001 |
| Bibliométrie | 0,001 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,001 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,001 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,002 | 0,001 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».