Multi-stressor impacts on fish energetics and stress response: a comparison between lab and field studies
Notice bibliographique
Résumé
Aquatic organisms are continuously exposed to multiple environmental stressors that work cumulatively and synergistically to alter ecosystems. The objective of this study was to investigate the impacts of multiple stressors under lab and field conditions using two fish species, zebrafish (Danio rerio) and rainbow darter (Etheostoma caeruleum). Under lab conditions, the study examined the impacts of chronic exposure to environmentally relevant concentrations of venlafaxine and elevated water temperatures on the energetics and stress response of zebrafish. Venlafaxine is a frequently prescribed antidepressant that is readily detectable in many Canadian waterways that receive discharged wastewater treatment plant effluent. Under field conditions, rainbow darter were collected upstream and downstream of the Waterloo wastewater treatment plant in the Grand River, Ontario, Canada. Enzyme activities of major metabolic and mitochondrial enzymes were measured in muscle tissue of both species. Oxygen consumption was measured to assess the effects of stressors on routine metabolic rate, active metabolic rate, aerobic scope, and critical swimming speed, all of which are significant indicators of fishes performance in ecological frameworks. Finally, the impact of chronic exposure to multiple stressors on the cortisol stress response following confinement and air-exposures stressors was investigated in zebrafish and rainbow darter respectively. \nIn the lab study, it was found that fish exposed to multiple stressors had higher oxygen consumption rates, resulting in diminished aerobic scope. No impairment in the cortisol stress response was observed in response to either stressors, singularly or cumulatively. Exposure to multiple stressors also had an impact on the enzyme activities in the muscle tissue of zebrafish. Fish exposed to multiple stressors had higher pyruvate kinase activity, reduced 3-hydroxyacyl CoA dehydrogenase temperature sensitivity, and lower catalase activity in response to the higher exposure temperature. \nIn the field study, it was found that male and female rainbow darter collected downstream of the Waterloo wastewater treatment plant had higher oxygen consumption rates. No impairment in the cortisol stress response between the downstream and upstream fish was observed, however, baseline cortisol levels in female fish from the downstream site were significantly higher compared to other baseline groups. Elevated cortisol levels were also higher in female fish from both sites when compared to their male counterparts. Differences in enzyme activities measured in the muscle tissue of rainbow darter were detected, however, differences were mostly associated with sex rather than collection site. \nThe results suggest that multi-stressor research can yield unexpected data that might not have been predicted using conventional single-stressor approach. Aligning field and lab results will highlight the importance of including multi-stressor approach assessments in making predictions regarding the impact of environmental stressors on non-target organisms.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».