Transcriptome profiling and identification of differentially expressed transcripts in response to mid season drought in groundnut Arachis hypogeae L
Notice bibliographique
Résumé
Drought is one of the major constraints in groundnut and its inheritance is governed by many genes with small effects operating in a coordinated manner. To this end, a total of eleven genotypes viz., ICGV 07070, ICGV 07132, TCGS 1398, TCGS 1073, TCGS 1157, TCGS 1173, MLTG 4, Narayani, Tirupati 1, Kadiri 6 and Kadiri 9 were screened in pot culture for moisture stress tolerance. Among the eleven genotypes, TCGS 1157 and MLTG 4 genotypes were more tolerant and Narayani and Kadiri 6 were highly sensitive to moisture stress. To unravel the molecular mechanisms conditioning drought tolerance, transcriptome was profiled cDNA-RAPD in these contrasting genotypes submitted to midseason moisture stress (50-80 DAS) and compared with respective well-watered control in field conditions. cDNA-RAPD profiles were developed with 35 RAPD markers in three regimes of moisture stress i.e., 60, 70 and 80 DAS. The transcript profile data revealed that drought stress induces the expression of many drought responsive transcripts and prolonged moisture stress has enormous impact on gene expression pattern. In the 35 cDNA- RAPD profiles analysed, a total of 823 transcripts were differentially expressed of which 523 transcripts exhibited qualitative difference (switched on: 263 transcripts, switched off: 260 transcripts) and 300 transcripts displayed quantitative differences (up regulated: 122 transcripts, down regulated: 178 transcripts). In the initial stages of moisture stress (10 to 20 days) many novel transcripts were activated along with modulation of gene expression (both up and down regulation) in comparison with well-watered control genotypes. The novel transcripts triggered by moisture stress will play a major role in stress perception, signal transduction, and synthesis of regulators and different compounds associated with drought tolerance mechanism. Under prolonged drought stress (30 days), the expression of novel transcripts were reduced by 30% and the up and down regulated transcripts was increased by 15%. Two moisture stress responsive transcripts were identified with OPA2 (750 bp) and OPA4 (450) markers. They were expressed only under drought stress in resistant genotypes (TCGS1157 and MLTG4) and absent susceptible genotypes and also in well-watered control of all the four genotypes three moisture stress regimes. Further characterization of these transcripts by sequencing will greatly help in understanding nature of the genes and the mechanism by which groundnut plants respond to drought stress. After validation, these two markers can be routinely employed for selection of drought tolerant germplasm lines.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,001 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,001 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».