194 Usefulness of the Ultrasound Imaging Modalities B-Mode and Colour Doppler Sonography and Serum Progesterone Concentrations for Determining Superovulatory Ovarian Responses in Ewes
Notice bibliographique
Résumé
The main goal of this study was to assess the usefulness of the 2 imaging modalities—B-mode and colour Doppler sonography—for determining the superovulatory response in ewes. In addition, serum concentrations of progesterone (P4) were examined for correlations with the numbers of laparoscopically detected luteal structures. Oestrus was synchronized in 24 sexually mature Santa Inês ewes with intravaginal P4-releasing devices (CIDR®) inserted for 8 days on a random day of the oestrous cycle. An injection of 37.5 µg of prostaglandin F2a analogue (d-cloprostenol) was given at the time of CIDR® insertion and withdrawal. The superovulatory treatment consisting of 8 consecutive doses of porcine FSH (total dose of 200 or 133 or 100 mg of pFSH; n = 8/total dose) given at 12-h intervals began 48 h before CIDR® removal. All animals received a single injection of 300 IU of eCG concurrently with a first dose of pFSH. On the day of surgical embryo recovery (6 days after the onset of behavioural oestrus), the ovaries of all donor ewes were visualised and examined with transrectal ultrasonography and then with videoendoscopy to identify and enumerate corpora lutea (CL) and luteinized unovulated follicles (LUF). Jugular blood samples were collected just before ovarian imaging for the measurement of circulating P4 concentrations. Ovaries could be easily and repeatedly detected with both the transrectal ultrasonography and videoendoscopic technique. Prematurely regressing CL could not be distinguished from healthy CL using either B-mode or colour Doppler ultrasonography but they were identified by their pale color by using videoendoscopy. All animals responded to the superovulatory treatment and produced =4 CL; however, a wide variation in ovarian responses was observed among ewes (range: 4–24 CL/ewe). Three ewes failed to produce healthy CL and had prematurely regressing CL only. In general, LUF were observed in 20/24 ewes (1–5/ewe) and 14/24 ewes had regressing CL (1–22/ewe). The total number of CL (r = 0.78 and 0.83; P < 0.0001) and LUF (r = 0.74 and 0.90; P < 0.0001) enumerated using the B-mode and colour Doppler ultrasonographic technique, respectively, were correlated with that ascertained by videoendoscopy. The determination of the superovulatory response was better by colour Doppler ultrasonography in relation to the B-mode, because it allows a better delimitation of the luteal structures. Serum P4 concentrations were related inversely to the numbers of prematurely regressing CL (r = –0.70; P = 0.0004) and directly to the number of healthy CL (r = 0.69; P = 0.0005). The present results indicate that ultrasonographic imaging and serum P4 measurements on the day of embryo recovery are both very accurate indicators of ovarian responses in superovulated ewes.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,001 | 0,001 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».