Biochemical characterization of Burkina red radish (<i>Raphanus sativus</i>) peroxidase
Notice bibliographique
Résumé
Objectives: Peroxidases (POX), isoenzymes were purified to homogeneity from bulbs of Raphanus sativus (Radish) grown in Burkina Faso and characterized for use as an alternative source of POX for biotechnological applications.Methodology and Results: The enzymes were purified using cation exchange, anion-exchange and hydrophobic interaction chromatography. Two isoperoxidases, cationic POX1 and anionic POX2, were isolated and purified 18.5 and 27.2 fold, respectively. Purified enzymes were found to be monomeric proteins with molecular masses of 70 kDa and 47 kDa for POX1 and POX2, respectively, as determined by SDS-PAGE. The effect of pH and temperature was done using guaicol as a substrate. The optimal pH of the both purified POX was 5.6 and 80 % of its activity was retained at pH values between 4.0–8.0 after incubation for 2 h, but POX2 appeared to be highly stable than POX1. POX1 and POX2 had optimum temperatures of activity at 35° and 40°C, respectively. Activities of these isoenzymes were determined using different phenolic substrates in the presence and absence of ionic effectors. The results show that both POX activities were activated by bivalent cations such as Mg2+ and Ca2+ but were inhibited by K+, Na+, Zn2+, EDTA and reducing compounds. While Ba2+ is an activator for POX2, it had no effect on POX1. They oxidize all the phenolic compounds used. The greatest rate was obtained with ABTS and guaiacol, for POX1 and POX2, respectively.Conclusions and application of findings: POXs oxidize a wide range of phenolic substrates and have high stability at a wide range of pH. These properties make these enzymes potential biotechnological tools. They could be use in industrial applications to produce food dyes, phenolic resins from natural phenolic compounds or in the construction of biosensors and kits for analyses and diagnostics. They could play also an important role in the oxidation of phenolic compounds in polluted water. This is interesting because it could be useful in finding solutions to the thorny problem of recalcitrant phenolic compounds that resist to conventional methods for bioremediation.Keywords: peroxidase, purification, characterization, Raphanus sativus, phenolic compound
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Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,001 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,001 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».