P003 C86/CD16 macrophages may act as a source of WNT2b in intestinal tissue from B3 Crohn’s disease patients
Notice bibliographique
Résumé
Macrophages contribute to fibrosis through the release of different mediators and the pattern of secretion may vary according to their phenotype. The expression of WNT ligands has been related with the macrophage phenotype and strong evidence identifies the WNT signalling pathway as an emerging modulator of fibrosis. The aim of the present study was to analyse the pattern of expression of macrophages and the expression of WNT ligands in surgical resections from Crohn’s disease (CD, n = 43) patients which were categorised according to Montreal classification (B2 or B3; unaffected mucosa of patients with colorectal cancer was used as control). mRNA was isolated from intestinal samples and the expression of macrophage markers and WNT2b was analysed by RT-PCR. The number of macrophages positive for the different markers (CD206, CD86, CD16, and WNT2b) was determined by flow cytometry. PBMCS were isolated from healthy donors and treated during 5 days with secretomes, from control, B2 or B3 surgical resections; the mRNA expression of macrophage markers and WNT2b was determined by RT-PCR. Intestinal crypts were isolated from control samples and were incubated for 24 h with WNT2b and the expression of EMT genes was analysed by RT-PCR. HT29 were treated for 7 days with WNT2b or TGFβ1 and immunofluorescence was performed. Results are expressed as mean ± SEM (n ≥ 5). Statistical analysis was performed by ANOVA + Newman–Keuls test. *p < 0.05 significant differences vs. Non-IBD group or vehicle, #p < 0.05 vs. B2-CD group. The expression of WNT2b was significantly higher in intestinal samples from B3 CD patients (2.3 ± 0.4) than in controls (1.1 ± 0.1) or B2 patients (0.7 ± 0.1). The number of CD16 or CD86-positive macrophages was significantly higher in intestinal tissue from B3 CD patients (69.7 ± 24.4% and 88.8 ± 18.4%, respectively) than in that from B2 CD patients (36.12 ± 5.8% and 30.58 ± 10.9%, respectively). A high percentage of CD16 positive macrophages in intestinal tissue from B3 CD patients were also positive for WNT2b (24.7 ± 8.8%). The mRNA expression of CD16, CD86, and WNT2b was significantly higher in PBMCS treated with B3-secretomes than in those treated with B2- or control secretomes (A). Exogenous administration of WNT2b to intestinal crypts induced the mRNA expression of EMT genes (B). WNT2b and TGFβ1-induced VIMENTIN expression in HT29 cells (C). Abstract P002 – WNT2b induces EMT. (A and B) Relative mRNA expression vs. β-ACTIN and represented as fold induction vs. vehicle-treated. (C) Images showing VIMENTIN and nuclear staining in HT29 cells. A macrophage phenotype expressing CD86/CD16 may act as a source of WNT2b in intestinal tissue from CD patients with a penetrating (B3) behaviour. WNT2b induces EMT in intestinal crypts and HT29 cells.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,005 | 0,001 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».