A13 PROTEINS AND FIBROSTENOTIC CROHN’S DISEASE; WHO SHOWED UP TO THE PARTY?
Notice bibliographique
Résumé
Crohn’s Disease (CD) is an inflammatory bowel disease mainly affecting the terminal ileum (TI) with three distinct phenotypes including strictures. Despite advances in medical therapy, fibrostenosis remains a therapeutic challenge as there are no available anti-fibrotic treatments. To date, there are no biomarkers to discriminate stricturing CD from other phenotypes. Early studies suggest that protein biomarkers identified from serum profiling may differentiate CD subtypes, and predict biologic therapy response. However, no studies have assessed which cell surface proteins are found in patients with strictures. Utilizing intestinal ultrasound (US), which readily detects fibrostenotic CD strictures, the serum of patients with and without strictures was collected for proteomic characterization. To investigate differences in the serum proteome among CD patients with fibrostenosis, as identified on bowel US, to those without strictures. All consecutive CD patients attending outpatient US appointments received greyscale US. Strictures were defined as a fixed bowel segment with a point of luminal apposition with or without prestenotic dilation. Five patients with ileal strictures were matched with similar ileal CD patients with only inflammatory behaviour, as confirmed on US. Patient sera was collected for quantitative shotgun proteomics using liquid chromatography/mass spectrometry. Statistical analysis was conducted at 1% false discovery rate to identify cell surface proteins to differentiate CD with and without strictures. 10 CD patient samples were collected; five patients allocated to the stricture and five to the non-stricture group. Demographics and age at diagnosis were similar between groups. Mean ileal bowel wall thickness was greater in the strictured (7.5 mm) group than without (4.5mm, p = 0.02). Prestenotic dilation (2 to 5 cm) was present in all with ileal stricture. All patients with a stricture had inflammation (mild/moderate inflammatory fat, hyperemia), while all but one in the non-strictured group had quiescent CD. More than 1,600 proteins were identified. In the strictured group, 4/5 patients had elevated Plexin-A2 and CD5 antigen-like protein, and 3/5 had elevated neogenin and dystonin. Of the non-strictured patients, 3/5 had elevated MMP-16/MT3-MMP (matrix metalloproteinase 16/membrane-type 3 MMP), and 4/5 had elevated vinculin. The sera of patients with and without structuring CD have a unique protein signature. These results provide an initial foundation driving further evaluation to confirm or refine candidate proteins, comparison to patients with resected strictures, and for mechanistic studies. Protein biomarkers may facilitate early stricture detection, monitor CD progression and ultimately identify patients in need of therapies with the greatest stricture risk. None
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».