HYDROGEN SULFIDE SYSTEM ATTENUATES INJURY BY HYPERGLYCEMIA AND STRESS: THE ROLE OF MESENTERIC ADIPOCYTES IN AGED ANIMALS
Notice bibliographique
Résumé
Introduction. The mesentery is one of recently described separate organs which functions and its ability to protect injury are still unclear. Adipocytes are a part of mesentery (MAC), however, it is a little known about their age-related changes, reactive response during damage induced by hyperglycemia, stress, and their interaction. Hydrogen Sulfide (H2S) system is key endogenous cytoprotectvite system that operated by catalytic activities of Cystathionine-γ-lyase (CSE), Cystathionine-β-synthase (CBS), and Sulfite Oxidase (SUOX) but their role in adipocyte cytoprotection are still unknown. Aim. To evaluate the CBS, CSE, SUOX activities in the mesenterium and ultrastructural changes of MAC in aged rats fed with HSD, at stress induction, and modification of H2S synthesis by NaHS and hybrid nonsteroid anti-inflammatory drugs H2S-acytylsalicylic acid (H2S-ASA). Material and Methods: All experiments were carried out on the aged rats which divided to the control group (standard diet) and experimental groups (28-days hypercaloric high-carbohydrate diet, as HSD by V. Kozar, 2008) without and with acute stress by water-immobilization stress model by Takagi, 1964, and pretreated per os by: 1) control group by vehicle (1,0 ml of saline), rats from 2-8 groups with HSD for 2) 1,0 ml of saline; 3) NaHS in a dose of 100 мkmol/kg, 9 days; 4) 1,0 ml of saline and induction of stress; 5) NaHS at a dose of 100 мkmol/kg and induction of stress; 6) ASA at a dose of 10 mg/kg/day for 9 days, NaHS at a dose of 100 мkmol/kg, and induction of stress; 7) ASA,10 mg/kg/day, per 9 days; 8) H2S-ASA ( ATB-340), 17,5 mg/kg/day, per 9 days). Subcellular changes of MAC were detected by transmission electron microscopy (TEM). MAC CSE, CBS, SUOX activities were evaluated by standard biochemical methods. Results. In HSD group with NaHS endothelial and sub-endothelial structures destructions were absent, the nucleus of endothelial cells it was observed an uneven condensation of chromatin. Effect of hybrid H2S-ASA characterized as a protective action and MAC “slimming”. The use of H2S-ASA showed an increase on 15% of the general activity of H2S/CSE and H2S/CBS vs ASA pretreatment (p<0.05). The use of aspirin and NaHS has shown an increase on 67% H2S/CSE and H2S/CBS activities in HSD rats vs saline group. SUOX activity was 5.27 nmol/min•mg in control, HSD rats – 4.05 nmol/min•mg; with NaHS HSD and stress were approximately same as control. Conclusions: This study suggests an important role for CSE/H2S pathway in the mesenteric adipocyte cellar survival and control cytoprotection. High expression activities in CSE/H2S and SUOX pathways in mesenteric adipocytes compromised by age, hyperglycemia might be associated with the development of low-grade inflammation and may be a potential therapeutic target for obesity treatment. H2S-ASA could be an effective tool for prevention of age-related inflammation and exert defensive effect against hyperglycemia.
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Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,001 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».