MP62-09 DETRUSOR RELAXATION MEDIATED BY ACTIVATION OF THE SUCCINATE RECEPTOR GPR91.
Notice bibliographique
Résumé
You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research & Pathophysiology (MP62)1 Apr 2019MP62-09 DETRUSOR RELAXATION MEDIATED BY ACTIVATION OF THE SUCCINATE RECEPTOR GPR91. Abubakr Mossa, Monica Velasquez-Flores, Philippe Cammisotto, and Lysanne Campeau* Abubakr MossaAbubakr Mossa More articles by this author , Monica Velasquez-FloresMonica Velasquez-Flores More articles by this author , Philippe CammisottoPhilippe Cammisotto More articles by this author , and Lysanne Campeau*Lysanne Campeau* More articles by this author View All Author Informationhttps://doi.org/10.1097/01.JU.0000556832.17091.fcAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVES: The bladder urothelium and detrusor layer express the succinate receptor, GPR91. The acute and immediate effects of its activation on bladder function have yet been described. The aim of this study is to demonstrate the acute effect of succinate activation of its receptor GPR91 on bladder contraction/relaxation using a mouse model with specific deletion of the GPR91 receptor. METHODS: Conscious cystometry was carried out on C57Bl6 and GPR91(-/-) mice with and without succinate intravesical instillation. Contractile properties of bladder strips from the 2 strains were analyzed by organ bath. Characteristics of bladder were analyzed by Masson's trichrome and immunoblotting. Primary urothelial cell culture from both groups were investigated for nitric oxide secretion (colorimetric assay), ATP and cGMP levels (commercial kits). RESULTS: The body weight was similar between GPR91(-/-) and C57Bl6 mice, while the bladder mass was higher in GPR91(-/-) mice. Masson's trichrome revealed a thicker urothelium and lamina propria in GPR91(-/-) mice. Conscious cystometry showed that GPR91(-/-) mice had a lower bladder capacity, lower micturition volume and lower intercontraction interval. Intravesical instillation of succinate (10 mM) increased the bladder capacity in C57Bl6 mice only. In organ bath, bladder strips from C57Bl6 and GPR91(-/-) mice had similar contractions elicited by potassium chloride ,carbachol and electrical field stimulation. Succinate administration relaxed carbachol-stimulated strips in C57Bl6 mice only. In vitro, short-term secretion of nitric oxide by urothelial cells from C57Bl6 and KO mice were similar at all concentrations (from 200 µM to 10 mM), confirming that nitric oxide is not involved in relaxation elicited by succinate. Succinate did not affect cGMP levels either in cell culture but decreased ATP released into the culture medium. CONCLUSIONS: These results suggest that succinate modulates the relaxation-contraction of the detrusor through its action on its receptor GPR91, possibly by decreasing ATP synthesis in the urothelium. Source of Funding: This research was funded by the FRQS. Montreal, Canada© 2019 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 201Issue Supplement 4April 2019Page: e890-e890 Advertisement Copyright & Permissions© 2019 by American Urological Association Education and Research, Inc.MetricsAuthor Information Abubakr Mossa More articles by this author Monica Velasquez-Flores More articles by this author Philippe Cammisotto More articles by this author Lysanne Campeau* More articles by this author Expand All Advertisement PDF downloadLoading ...
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,001 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,001 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,001 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,031 | 0,005 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».