MP75-07 SOX2, A MASTER REGULATOR OF STEMNESS, IS OVEREXPRESSED IN TESTICULAR TISSUE OF MEN WITH SERTOLI CELL ONLY SYNDROME
Notice bibliographique
Résumé
You have accessJournal of UrologyInfertility: Basic Research & Pathophysiology (MP75)1 Apr 2019MP75-07 SOX2, A MASTER REGULATOR OF STEMNESS, IS OVEREXPRESSED IN TESTICULAR TISSUE OF MEN WITH SERTOLI CELL ONLY SYNDROME Russell Hayden*, Anna Mielnik, Ryan Flannigan, Peter Schlegel, and Darius Paduch Russell Hayden*Russell Hayden* More articles by this author , Anna MielnikAnna Mielnik More articles by this author , Ryan FlanniganRyan Flannigan More articles by this author , Peter SchlegelPeter Schlegel More articles by this author , and Darius PaduchDarius Paduch More articles by this author View All Author Informationhttps://doi.org/10.1097/01.JU.0000557251.15903.57AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVES: The SOX family of transcription factors play critical roles in development, stem cell maintenance, and cellular differentiation. SOX2 is one of the 4 Yamanaka factors, which also include OCT4, KLF4, and MYC, that are used to induce pluripotent stem cells. SOX2 is normally not expressed in the testis, in contrast to SOX3 which localizes to spermatogonial stem cells. However, preliminary RNA-sequencing results in our lab demonstrated expression of SOX2 in men with Sertoli Cell Only Syndrome (SCO). In this study we sought to substantiate these results with real time PCR (RT-PCR). METHODS: Testis biopsies were obtained during testicular sperm extraction for infertility. RNA-seq was performed on testis biopsies from 11 men with SCO and 10 with normal spermatogenesis using the Illumina HiSeq2000 platform. Reads were mapped using the STAR Aligner v2.5 against human genome hg38. Raw counts were normalized with Limma v3.6 using the R statistical package v3.4. RT-PCR was conducted on 3 men with SCO and 3 men with normal spermatogenesis on a LightCycler 480 (Roche). The RT2 qPCR platform was used in all experiments (Qiagen), and all assays were conducted in triplicate. Quality control was assured using melting point analysis, interplate calibrators, and negative controls. RESULTS: RNA-seq data demonstrated a 6-fold (p < 0.01) increase of SOX2 expression in men with SCO as compared to normal controls. The mean counts per million (CPM) for men with SCO was 46.5 (CI 35.2 - 57.8), whereas men with normal spermatogenesis had a CPM value of 7.8 (CI 5.9 - 9.8). RT-PCR results demonstrated a 2.7-fold (CI 1.7 - 4.1) increase in SOX2 expression of SCO compared to normal controls. CONCLUSIONS: SOX2 is over expressed in men with SCO as compared to normal controls. Although RT-PCR produced an attenuated result, the fold change in expression levels between SCO and normal men remained significant. Further study is needed to delineate how SOX2 overexpression may contribute to the pathophysiology of SCO, especially regarding its ability to suppress stem cell differentiation. Source of Funding: P50 HD076210, U1 1U01HD074542-01; Frederick J. and Theresa Dow Wallace Fund of the New York Community Trust, the Mr. Robert S. Dow Foundation; Irena and Howard Laks Foundation. New York, NY; Vancouver, CanadaNew York, NY© 2019 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 201Issue Supplement 4April 2019Page: e1084-e1084 Advertisement Copyright & Permissions© 2019 by American Urological Association Education and Research, Inc.MetricsAuthor Information Russell Hayden* More articles by this author Anna Mielnik More articles by this author Ryan Flannigan More articles by this author Peter Schlegel More articles by this author Darius Paduch More articles by this author Expand All Advertisement PDF downloadLoading ...
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,001 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,009 | 0,001 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».