SAT-360 Acute Stress Increases Local Corticosterone Levels In Lymphoid Organs Of Neonatal Mice: Analysis Using LC-MS/MS
Notice bibliographique
Résumé
Glucocorticoids (GCs) are steroids produced by the adrenal glands and also by lymphoid organs such as bone marrow, thymus, and spleen (Taves et al., 2015). GCs are critical regulators of immune system development. During early development (postnatal day (PND) 2 to 12), mice show decreased adrenal GC secretion at baseline and in response to stressors, which is termed the stress hyporesponsive period (SHRP) (D’Amato et al., 1992). Lymphoid organs locally produce GCs, particularly during the SHRP, suggesting that these organs might increase local GC production in response to stress. Here, using PND1, PND5, PND9, and PND13 mice, we administered 5% isoflurane (an anesthetic) in 21% oxygen as an acute stressor, 21% oxygen as a vehicle control, or neither (baseline). For both isoflurane and oxygen treated groups, pups were removed from their home cage and placed in an induction chamber with a heating pad and nesting material from their home cage. Animals were given either given isoflurane for 3min followed by 27min oxygen or 30min continuous oxygen and rapidly euthanized. Baseline animals were given neither isoflurane nor oxygen and were rapidly euthanized in less than 3min. We then measured a panel of 7 steroids, including corticosterone, in the blood and lymphoid organs using liquid chromatography tandem mass spectrometry (LC-MS/MS). Note that PND1 is pre-SHRP, PND5 and PND9 are within the SHRP, and PND13 is post-SHRP. At PND1, corticosterone levels were generally high in both blood and lymphoid organs and did not differ with treatment. At PND5, corticosterone levels were generally very low, but increased with stress in a tissue-dependent fashion, showing the greatest increase in bone marrow and the least increase in blood. At PND9, baseline corticosterone levels were very low, but increased in both blood and lymphoid organs in response to a stressor. At PND13, corticosterone levels were higher in blood than in lymphoid organs for all treatments and increased with stress. Taken together, these novel data indicate that during the SHRP, an acute stressor produces little to no increase in corticosterone levels in blood but produces a 100-fold increase in bone marrow and a 20-fold increase in thymus and spleen. These data support the exciting possibility that mouse lymphoid organs can locally produce corticosterone, even when the adrenal glands produce little corticosterone. More generally, it is important to recognize that local steroid levels often do not match systemic steroid levels and that local steroid synthesis is particularly important in certain developmental and physiological contexts.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,001 | 0,001 |
| Bibliométrie | 0,000 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».