Identification of new microbial functional standards for soil quality assessment

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Catégories consensuelles

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Domaine

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Devis d'étude

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Genre

Signal candidat: EmpiriqueSignal consensuel: Empirique

Score de désaccord entre enseignants

0,867

Score d'incertitude au seuil

0,136

Statut de validation

machine_predicted_unvalidated · codex-gemma-dda1882f352a

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Écart entre enseignants

0,243 · la distance entre les deux têtes enseignantes sur ce seul travail

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Résumé

Abstract. The activity of microorganisms in soil is important for a robust functioning of soil and related ecosystem services. Hence, there is a necessity to identify the composition, diversity, and function of the soil microbiome in order to determine its natural properties, functioning, and operating range as well as to assess ecotoxicological effects due to anthropogenic activities. Numerous microbiological methods currently exist in the literature and new, more advanced methods continue to be developed; however, only a limited number of these methods are standardised. Consequently, there is a need to identify the most promising non-standardised methods for assessing soil quality and to transform them into standards. In agreement with the “Ecosystem Service Approach”, new methods should focus more on soil microbial functions, including nutrient cycling and greenhouse gas emission, pest control and plant growth promotion, carbon cycling and sequestration, as well as soil structure development and filter function. The few existing standardised methods available that focus on the function of the soil microbiome mostly include measurements, like basal respiration, enzyme activities, and biodegradation of organic matter, under well-defined conditions in the lab. This paper sets out to summarise and expand on recent discussions within the International Organization for Standardization (ISO), Soil Quality – Biological Characterization sub-committee (ISO TC 190/SC 4), where a need was identified to develop scientifically sound methods which would best fulfil the practical needs of future users for assessing soil quality, going beyond the existing test systems. Of particular note is the current evolution of molecular methods in microbial ecology that use quantitative real-time PCR (qPCR) to produce a large number of new functional endpoints which are more sensitive as compared to “classical” methods. Quantitative PCR assesses the abundance of microbes that catalyse major transformation steps in nitrogen and phosphorus cycling, greenhouse gas emissions, chemical transformations including pesticide degradation, and plant growth promotion pathways based on the assessment of marker gene sequences that drive the related processes. In the assessment of soil quality methods, it was found that most methods focus on bacteria and related endpoints. Techniques to describe fungal communities as well as their functional traits are far less represented. As such, techniques to analyse fungal enzyme activities are proposed. Additionally, methods for the determination of microbial growth rates and efficiencies, including the use of glomalin as a biochemical marker for soil aggregation, are discussed. Furthermore, field methods indicative of carbon turnover, including the litter bag test and a modification to the tea bag test, are presented. However, it is obvious that with increasing developments in high throughput sequencing technologies and big data analyses, including metagenomics analysis, it will be possible to implement these technologies into the standardisation process for assessing the functions of the soil microbiome. Overall, it is suggested that endpoints should represent a potential function of soil microorganisms rather than actual activity levels, as the latter can largely be dependent on short-term variable soil properties such as pedoclimatic conditions, nutrient availability, and anthropogenic soil cultivation activities.

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La notice

Revue

SOIL

Thématique

Soil Carbon and Nitrogen Dynamics

Domaine

Agricultural and Biological Sciences

Établissements canadiens

Environment and Climate Change Canada

Organismes subventionnaires

Analyses et Expérimentations pour les EcosystèmesAgence Nationale de la Recherche

Mots-clés

Environmental scienceSoil qualitySoil functionsStandardizationEnvironmental resource managementEcosystem servicesSoil organic matterSoil healthBiochemical engineeringComputer scienceEcologyEcosystemSoil waterSoil biodiversityEngineeringBiologySoil science

Résumé présent dans OpenAlex

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