Yeast population and community dynamics : their effect on the chemical and sensory profiles of inoculated and spontaneous Pinot noir and Chardonnay fermentations at a Canadian winery
Notice bibliographique
Résumé
The Okanagan Valley of British Columbia, Canada, has grown to include over 130 wineries, spanning 8,060 acres. As a result, competition within the local industry and has caused Okanagan winemakers to seek the production of a more sensorially unique product. Traditionally, inoculated fermentations are most commonly conducted by adding an abundance of a Saccharomyces cerevisiae yeast strain to produce consistent and specific chemical and sensory attributes in the wines; however, winemakers are choosing to showcase their unique winery yeast flora by conducting spontaneous fermentations. Spontaneous fermentations are conducted under the assumption that the fermentative yeast populations come from diverse winery resident organisms which, therefore, create different chemical and sensory profiles. Specifically, spontaneous and inoculated fermentations of Pinot noir and Chardonnay during the 2013 vintage at Quails’ Gate Estate Winery were assessed. Throughout fermentation these wines were subjected to both culture-dependent microsatellite analysis and isolate sequencing, as well as culture-independent Illumina MiSeq analysis. This determined the yeast populations conducting alcoholic fermentation and compared the capabilities of each method when studying yeast communities. Uniquely diverse yeast populations were found between spontaneous and inoculated fermentations of Pinot noir; however, yeast populations within inoculated and spontaneous Chardonnay treatments showed no differentiation. Unexpectedly, Pinot noir and Chardonnay fermentations were found to include non-Saccharomyces yeasts, specifically Hanseniaspora sp., throughout the entirety of fermentation in large relative abundance. Gas chromatography flame ionization detection (GC-FID) was conducted to determine the concentration differences of fermentation-derived chemicals associated with the identified yeast species fermenting both varietals; however, only isoamyl acetate, was found to differ between Pinot noir treatments. Sensory analysis revealed unique attributes were associated with the sensory profiles of inoculated or spontaneously fermented Pinot noir wines. The unique yeast communities found between inoculated and spontaneous fermentations of Pinot noir were responsible for slight chemical variation and unique sensory profiles between treatments. The similarity in yeast communities between inoculated and spontaneous fermentations of Chardonnay precluded chemical and sensory differences from occurring. Thus, to increase the chemical and sensory variation within wines and differentiate varietals from those fermented by competitors, wineries should consider producing both inoculated and spontaneous fermentations.
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Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».