Anti-Proliferative and Apoptotic Activity of Rumex obtusifolius seed’s Hydroalcoholicextract on Human Colon Cancer Cell Line “SW480”
Notice bibliographique
Résumé
Cancer is considered a serious threat to human health thatcolon cancer is the third most common cancer in the world. Colon cancer has undergone significant growth worldwide in recent years, and many people die every year as a result of this disease, which its treatment is a major challenge for researchers. In this study, we investigated the effect of anti-proliferative and apoptotic activity seed’s hydroalcoholic extract of Rumex obtusifolius, “is native to Semnan's northern region” on the human colon cancer cell line, SW480. The SW480 human colon cancer cell line were cultured in RPMI 1640 cell culture containing 10% Fetal bovine Serum and 1% Penicillin and Streptomycin antibiotics and incubated with a relative humidity of 95%, carbon dioxide 5% at 37°C. The SW480 cancer cell line was cultured in 48 and 96 well plates and treated with different concentrations of seeds hydroalcoholic extract of Rumex obtusifolius (20/40/80/160/320 micrograms/ml) for evaluation of cell viability by Trypan blue exclusion method and MTT assay. A non-treated group was used as control and a treated group with DMSO was used as solvent in solution of powdered extract as vehicle as well as positive control groups. Also, cell apoptosis assessment was performed by Propideum iodide/Annexin V test “Flowcytometry”and DNA fragmentation analysis “Agaros electrophoresis”. Seed hydroalcoholic extract caused cell death at concentrations of 40, 80, 160 and 320μg/ml, which the anticancer effect trend to increase significantly from 80μg/ml. The effect of R. obtusifolius seed extract even has more anti-cancer effect in 160 and 320μg/ml than cells which is treated to 5FU as a positive control groups. Seed hydroalcoholic extract reduced cell viability in proportion concentration. The effect of anticancer activity of seed extract trend to increase from 40μg/ml but it was shown effective concentrations for inhibiting of the cells growth were 160, 320μg/ml in 48 hr incubation time and 80, 160 and 320μg/ml in 72 hr incubation time. Also, the effect of this extract at concentrations of 160 and 320μg/ml with control group is not significant which have more cytotoxicity and even extract can have a significant effect on decreasing the survival of the SW480 cells. The data generated by flow cytometry are shown that colon cancer cells trend to necrosis and apoptotic pathway from 40μg/ml and increased significantly entrance to apoptotic pathway from 80μg/ml which is confirm the Trypan blue and MTT assay tests. Since the appearance of the DNA fragmentation, which precedes the bioavailability change, is characteristic to apoptosis, the extracts were considered to be apoptosis-inducers. The ability to inhibit growth and induction of apoptosis in SW480 cancer cells is a concentration- and time dependent manner. These results indicated that the induction of apoptosis at least partly mediates their cytotoxic activity which is necessary down further study for elucidation apoptotic signaling pathway.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,001 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».