Isolation and Characterization of Mesenchymal Stromal Cells from the Visceral Adipose Tissue in Peripancreatic Region
Notice bibliographique
Résumé
Type 1 Diabetes Mellitus (T1DM) is a multigenic autoimmune disorder that leads to the destruction of insulin producing β-cells of the pancreas by the host immune system. This can lead to chronic hyperglycemia, diabetic ketoacidosis (DKA), retinopathy, nephropathy, neuropathy, serious cardiovascular complications, severe hypoglycemic unawareness and glucose instability. Conventional management therapy includes daily glucose monitoring and exogenous insulin injections. Islet transplantation is an attractive alternative to conventional therapy. First attempts of islet transplantation dates to 1972. Clinical feasibility and efficacy of islet transplantation was first demonstrated by Edmonton protocol (EP) developed by the Islet Transplant Group in Edmonton in 2000. However, some of the limitations of this approach include limited islet supply, gradual graft loss, and harmful chronic immunosuppression regimen. Even with marked improvements, at its current state, the procedure it is reserved for specific subset of T1DM patients that have unstable T1DM and hypoglycemia unawareness, severe hypoglycemic episodes and glycemic lability that cannot be controlled with intensive insulin therapies. Some of the major limitations of islet transplantation that need to be overcome for it to be widely available are limited islet supply, chronic immunosuppression to prevent allograft rejection, and gradual graft loss. The last two could be potentially be addressed with Mesenchymal Stromal Cells (MSCs) which are multipotent stem cells found in the stroma of most of the tissues in the body. These cells have self-renewal capacity and can be differentiate into adipocytes, osteoblasts, and chondrocytes. MSCs can suppress the inflammation and promote tissue repair and regeneration through the secretion of cytokines, anti-oxidants, pro-angiogenic factors, anti-apoptotic factors, antimicrobial factors, and trophic molecules. Current literature suggests that there are source-dependent differences in MSCs with respect to cell yield per mass of tissue, transcriptome and secretome profiles, and proliferative and mitotic capacities. This thesis examined the ideas of microenvironment-dependent differences among different types of MSCs and that MSCs that are ontologically and anatomically closer to the islets might be more beneficial to them. Therefore, in this study we isolated and characterized cells from the visceral adipose tissue specifically in the peripancreatic region. In accordance with International Society for Cellular Therapy (ISCT), my data demonstrate that cells I prepared attached to the plastic, expressed a MSC-defining cell surface markers, and differentiated into adipocytes but not chondroblasts and osteoblasts. Reduced differentiation potential could be explained by the fact that the donors were elderly and obese, and the cells have undergone many mitotic divisions before undergoing differentiation protocols. Given the results from multiply analyses we denoted them as ppaMSCs. This newly characterized cells could be used in the future studies to assess their effects in islets transplantation.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».