Characterization and Evaluation of Submicron Femtosecond Laser-Induced Periodic Surface Structures on Titanium to Improve Osseointegration of Dental and Orthopaedic Implants
Notice bibliographique
Résumé
Surface properties such as topography and wettability play a pivotal role in controlling the cellular behavior on dental and orthopaedic implants and eventually their clinical success. The implementation of more advanced cell-targeted surface modification approaches has opened up additional possibilities for improving osseointegration to further increase the success rate of bone implants. In this thesis, the potential of employing femtosecond laser-induced periodic surface structures with submicron spatial periodicities of 300 nm, 600 nm and 760 nm on titanium to improve osseointegration of dental and orthopaedic implants was explored. Uniform submicron femtosecond laser-induced periodic surface structures with consistent periodicity, roughness and oxide thickness were generated over large areas (10 x 10 mm^2) on titanium substrates and characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), electron energy loss spectroscopy (EELS), and Auger electron microscopy (AES). In vitro experiments using osteosarcoma Saos-2 cells showed the same level of cell metabolism on the laser textured and unmodified (control) surfaces along with statistically significant alkaline phosphatase activity after 14 days of cell seeding for the laser patterned surface with periodicity of 620 nm compared to the control surface. Average circularity along with nuclear area factor of cells fixed onto the laser textured and unmodified titanium surfaces were acquired from SEM images using ImageJ. The lower circularity and higher nuclear area factor of cells was observed on all laser textured surfaces as compared to the control, and are indicative of healthier cells on the laser textured surfaces. The cells appeared to align perpendicularly to the periodic laser generated structures and showed a more elongated shape on laser patterned surfaces as compared with the control surface, with the cell’s filopodia appearing to be attached to the peaks of the laser-textured pattern. In the second part of the thesis, the mechanism underlying the wettability transition from superhydrophilic to superhydrophobic on femtosecond laser generated periodic surface structures on titanium was investigated. The time-dependent wettability of the laser treated surfaces was assessed by the sessile drop method. The samples exhibited superhydrophilic behavior immediately after laser texturing and became superhydrophobic over time. Detailed surface chemical analyses by X-ray photoelectron spectroscopy revealed that the unique electronic structures of Ti2O3 and TiO2, which resulted in hydrophilic and hydrophobic hydration structures, respectively, played a crucial role in the observed wettability transition. This study demonstrates the prospect of using femtosecond laser-induced periodic surface structures as a promising surface modification strategy to potentially manipulate cellular behavior and improve dental and orthopaedic implants’ clinical success rate.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».