Abstract P3-02-02: CD68-positive crown-like structures of the breast are independently associated with adverse survival: A retrospective analysis of cases from a prospective cohort
Notice bibliographique
Résumé
Abstract Introduction: The association of breast cancer (BC) with obesity is complex and multifactorial, depending partly on diet and related metabolic imbalances. Previous reports have shown that local inflammation in adipocytes can be seen in tissue sections as rings of macrophages of macrophages around necrotic adipocytes (“Crown-Like Structures of the Breast”, CLS-B). We and others have shown that CLS-B have higher prevalence and density within the adipose tissue of obese patients. Our goal was to examine the association of CLS-B with survival in participants enrolled at one center (Mount Sinai Hospital, Toronto) of a multicenter prospective cohort study designed to investigate obesity-related prognostic factors in early stage BC. Design: Archived H&E sections of breast adipose tissue were retrieved from Mount Sinai (Toronto) participants (N=163). All specimens were from breast excisions for invasive carcinoma. A CLS-B was defined as a ring of macrophages surrounding an adipocyte in otherwise normal breast tissue. In a subset (N=119), immunostains for CD68 were performed on a representative block to highlight macrophages. Sections containing tumor, fat necrosis, and mastitis were excluded. Slides were pathologist-reviewed, recording the number of CLS-B, slides containing CLS-B, and slides with normal tissue. Serologic markers of metabolism and inflammation were previously performed in a central laboratory. Correlations with clinical and serologic markers were examined using descriptive statistics and Wilcoxon tests. Survival analysis was performed with Cox proportional hazards models. Results: CLS-B were identified in routine H&E sections of normal fat in 59 out of 163 cases (36%). In the representative subset stained for CD68 (N=119), 22 cases were identified as positive for CLS-B by both H&E and CD68, 15 cases by CD68 alone and 24 by H&E alone, giving a CLS-B prevalence of 39% as detected by H&E, 31% by CD68 and 51% when combining the two. Both i) CD68-positive and ii) H&E-positive CLS-B showed similar positive relationships with BMI (median BMI 27 versus 24 kg/m2, P<0.01 for CLS-B present versus absent); however the relationship with insulin was stronger for CD68-positive CLS-B (median insulin 49 vs 35 pmol/L, P=0.009) than for H&E-positive CLS-B (insulin 39 vs 35 pmol/L, P=0.39). Univariable Cox model hazard ratios (HRs) for CD68-positive CLS-B were 2.79 (95% confidence interval (CI) 1.31-5.95) and 3.74 (CI 1.73-8.07) for disease-free and overall survival respectively, and after adjustment for age, nodal status, tumour grade, stage, ER/PR status, adjuvant treatment and BMI, HRs were 2.43 (CI 1.01-5.81) and 3.19 (CI 1.3-7.79). In the same subset, H&E-positive CLS-B had univariable HRs of 0.66 (CI 0.29-1.52) and 1.18 (CI 0.55-2.54) respectively and adjusted HRs of 0.31 (CI 0.11-0.82) and 0.57 (CI 0.23-1.39). Combining H&E and CD68 did not improve prediction over using CD68 alone. Conclusion: CLS-B visualized by the macrophage immunostain CD68 were associated with poor outcome, independent of patient BMI and tumour characteristics. Detection of CLS-B using H&E alone was not associated with poor outcome. Our results demonstrate that although CLS-B are a phenotype of obesity, they may also reflect a tissue-specific risk of adverse BC outcome. This risk may relate to localized tissue inflammation with higher prevalence in obese individuals. Although we do not advocate the clinical use of the CD68 stain, further work is warranted to clarify the connection between tissue inflammation and poor breast cancer outcome. Acknowledgement: The authors wish to acknowledge the Hold'Em For Life Charity Challenge for Cancer Research for their generous support. Citation Format: Martin C. Chang, Marguerite Ennis, Zohreh Eslami, Pamela J. Goodwin. CD68-positive crown-like structures of the breast are independently associated with adverse survival: A retrospective analysis of cases from a prospective cohort [abstract]. In: Proceedings of the 2019 San Antonio Breast Cancer Symposium; 2019 Dec 10-14; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2020;80(4 Suppl):Abstract nr P3-02-02.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,001 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,001 | 0,001 |
| Études des sciences et des technologies | 0,001 | 0,001 |
| Communication savante | 0,001 | 0,000 |
| Science ouverte | 0,001 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,003 | 0,001 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».