First Report of Leaf Spot Caused by <i>Colletotrichum fructicola</i> on <i>Callerya speciosa</i> (<i>Millettia speciosa</i>) in Guangxi, China

HomePlant DiseaseVol. 104, No. 12First Report of Leaf Spot Caused by Colletotrichum fructicola on Callerya speciosa (Millettia speciosa) in Guangxi, China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Leaf Spot Caused by Colletotrichum fructicola on Callerya speciosa (Millettia speciosa) in Guangxi, ChinaJuan Shu, Ping Ning, Tangxun Guo, Lihua Tang, Suiping Huang, Qili Li, Jianyou Mo, Zhihe Yu, and Tom HsiangJuan ShuCollege of Life Sciences, Yangtze University, Jingzhou, Hubei, 434025, ChinaInstitute of Plant Protection, Guangxi Academy of Agricultural Sciences and Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning, Guangxi, 530007, China, Ping NingGuangxi Agricultural Vocational College, Nanning, Guangxi, China, Tangxun GuoInstitute of Plant Protection, Guangxi Academy of Agricultural Sciences and Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning, Guangxi, 530007, China, Lihua Tanghttp://orcid.org/0000-0001-5972-0166Institute of Plant Protection, Guangxi Academy of Agricultural Sciences and Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning, Guangxi, 530007, China, Suiping HuangInstitute of Plant Protection, Guangxi Academy of Agricultural Sciences and Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning, Guangxi, 530007, China, Qili Li†Corresponding authors: Q. L. Li; E-mail Address: 65615384@qq.com and Z. H. Yu; E-mail Address: zhiheyu@hotmail.comhttp://orcid.org/0000-0001-5775-8910Institute of Plant Protection, Guangxi Academy of Agricultural Sciences and Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning, Guangxi, 530007, China, Jianyou MoInstitute of Plant Protection, Guangxi Academy of Agricultural Sciences and Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning, Guangxi, 530007, China, Zhihe Yu†Corresponding authors: Q. L. Li; E-mail Address: 65615384@qq.com and Z. H. Yu; E-mail Address: zhiheyu@hotmail.comCollege of Life Sciences, Yangtze University, Jingzhou, Hubei, 434025, China, and Tom HsiangSchool of Environmental Sciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada AffiliationsAuthors and Affiliations Juan Shu1 2 Ping Ning3 Tangxun Guo2 Lihua Tang2 Suiping Huang2 Qili Li2 † Jianyou Mo2 Zhihe Yu1 † Tom Hsiang4 1College of Life Sciences, Yangtze University, Jingzhou, Hubei, 434025, China 2Institute of Plant Protection, Guangxi Academy of Agricultural Sciences and Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning, Guangxi, 530007, China 3Guangxi Agricultural Vocational College, Nanning, Guangxi, China 4School of Environmental Sciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada Published Online:13 Oct 2020https://doi.org/10.1094/PDIS-02-20-0312-PDNAboutSectionsView articlePDFPDF PlusSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleCallerya speciosa Champ is a climbing shrub in the Fabaceae. It is widely grown in Guangxi, Guangdong, and Hainan provinces in China and so on, and it is widely cultivated for its tubers, which are edible and are reported to have antibacterial and antioxidant effects (Yin et al. 2010). In September 2014, leaf spot symptoms were observed on foliage of C. speciosa in a plantation in Nanning, Guangxi, China. In a 1,300-m2 cultivated area in Guangxi Agricultural Vocational College, there was a more than 50% incidence of leaf spots in the 700 two-year-old plants in this field. The disease severity varied between plants; some leaves showed small spots, and some young leaves were entirely blighted. Initial symptoms appeared as greenish yellow foliar spots, which turned brown and developed into circular, diamond, or elliptical shaped lesions, surrounded by yellow halos. Numerous acervuli were observed in the lesions at later stages. Acervuli were mainly epiphyllous, with two to three dark brown septate acicular setae. Diseased 2-mm-long leaf segments were surface sterilized in 75% ethanol for 30 s, in 0.1% HgCl2 for 30 s, rinsed with sterile distilled water, and plated on potato dextrose agar (PDA). A single Colletotrichum species morphotype was consistently recovered, with an 85% isolation rate from among 20 leaf pieces. On PDA, the colonies were white to light gray and black brown with abundant aerial hyphae. A gelatinous layer of conidiogenous cells not delimited in obvious sporodochia and lacking setae was observed developing on portions of the colonies forming on PDA plates. Conidia were elliptical, aseptate, and hyaline, 13.8 ± 0.2 × 6.5 ± 0.1 μm (n = 100). A single-spore isolate, NDL-3, was selected for molecular identification. The rDNA internal transcribed spacer region (ITS), actin (ACT), Apn2-Mat1-2 intergenic spacer and partial mating type (Mat1-2) (ApMat), calmodulin (CAL), chitin synthase (CHS-1), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes (Weir et al. 2012) were sequenced (deposited in GenBank as accession nos. MH721408, MH719084, MK610437, MK636569, MK592616, and MK592617, respectively). Phylogenetic analysis of NDL-3 by MrBayes (version 3.2.6) based on a concatenation of multiple genes sequences (ITS, ACT, CAL, CHS-1, and GAPDH) and the ApMat gene locus plus related sequences obtained from GenBank revealed that the isolate was C. fructicola (Weir et al. 2012). Pathogenicity tests were conducted on 30 attached artificially wounded leaves of three young healthy 2-year-old plants with 20-µl droplets of conidial suspension (106 spores/ml) per wound site. Wounded leaves were also mock inoculated with 20-µl droplets of water as controls. Inoculated plants were sprayed with water and throughout the incubation were always covered with plastic bags. After 2 weeks of incubation at 28°C, typical symptoms were seen on inoculated leaves but none on control leaves. Koch's postulates were fulfilled by reisolation of C. fructicola from diseased leaves. Although C. fructicola is known to occur on many different hosts, this is the first report of C. fructicola infecting C. speciosa in China. This research may accelerate the development of future epidemiological studies and management strategies for anthracnose caused by C. fructicola on C. speciosa.The author(s) declare no conflict of interest.References:Weir, B. S., et al. 2012. Stud. Mycol. 73:115. https://doi.org/10.3114/sim0011 Crossref, ISI, Google ScholarYin, T., et al. 2010. Fitoterapia 81:274. https://doi.org/10.1016/j.fitote.2009.09.013 Crossref, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: The research was supported by Guangxi Natural Science Foundation (2017GXNSFBA198218).DetailsFiguresLiterature CitedRelated Vol. 104, No. 12 December 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionUredinia of Phragmidium violaceum on European blackberry (K. J. Evans et al.). Photo credit: L. Morin. Strawberry fruit rot caused by Sclerotinia sclerotiorum (M. V. Marin and N. A. Peres). Photo credit: M. V. Marin. Metrics Downloaded 415 times Article History Issue Date: 1 Dec 2020Published: 13 Oct 2020First Look: 7 Jul 2020Accepted: 5 Jul 2020 Pages: 3256-3256 Information© 2020 The American Phytopathological SocietyFundingGuangxi Natural Science FoundationGrant/Award Number: 2017GXNSFBA198218Keywordsleaf spotColletotrichum fructicolaMillettia speciosaThe author(s) declare no conflict of interest.

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