Mechanically Dynamic Scaffolds for Enhanced Anterior Cruciate Ligament Reconstruction
Notice bibliographique
Résumé
Close to a quarter of a million ACL injuries occur each year in Canada and the United States. These injuries can result in reduced functional performance and are associated with long-term clinical conditions that include meniscal tears, chondral lesions and an increased risk of early onset osteoarthritis (OA). Surgical reconstruction is the current gold standard of care for ACL injuries. However, surgical treatment is costly and associated with high risk of post-traumatic OA within two decades of injury. Researchers have explored tissue engineering (TE) to address these shortcomings and have developed degradable graft materials that can guide functional connective tissue formation. However, scaffolds fabricated using TE methods still do not accurately mimic the anisotropic architecture and biomechanical properties of the ACL. Furthermore, the biological graft remodeling process causes significant reductions in tensile properties of the tissue-engineered scaffolds, which adds complexity to the design of such structures. Our hypothesis is that reductions in mechanical properties can be minimized by enclosing the scaffold in an artificial semi-permeable membrane and incubating it in vivo prior to ACL surgical reconstruction. A low-cost ACL scaffold was additively manufactured via fused deposition modeling using LAY-FOMM material (thermoplastic polyurethanes co-polymer with polyvinyl alcohol). Scaffolds replicating the anatomical dimensions of the ACL were printed on a Flashforge Creator Pro 3D Printer and evaluated for mechanical properties such as tensile strength, strain at failure, compression strength and Young's modulus. Second, the scaffolds will be soaked in a solution containing bFGF and TGF- growth factors and seeded with human adipose derived mesenchymal stem cells (ADSCs). The scaffolds will then be placed in a semi-permeable membrane and cells will be investigated for viability via a live/dead analysis and metabolic activity. Cell phenotype will be characterized using western blot and PCR to assess collagen types I and III, fibronectin and tenascin. Lastly, the impact of mechanical stimuli on tissue formation will be investigated using the Mechanoculture T6 bioreactor. There is currently a need for tissue-engineered ACL scaffolds that can appropriately mimic the biomechanical properties of the ACL and guide ligament formation. Due to the nature of the biological processes involved in graft reconstruction, most grafts undergo a significant reduction in tensile properties. The incubation of graft material prior to surgical intervention could maximize mechanical stability and allow patients to go back to regular activities faster with lower risk of recurring injuries.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,001 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».