80 Energetic substrate availability affects the metabolome profile in bovine sperm
Notice bibliographique
Résumé
Bovine spermatozoa are specialised cells that require high ATP production for flagellar movement and other physiological events necessary for fertilization. Glycolysis and oxidative phosphorylation (OXPHOS) are the most studied energy pathways in sperm cells and involve metabolites such as glucose, pyruvate, and lactate. Although glycolysis has been described as the preferential pathway for ATP generation in bovine spermatozoa, other metabolites may also be used, leading to distinct metabolome profiles. Thus, the objective of this work was to characterise the metabolome profile of culture media derived from sperm cells incubated in the presence of different energy substrates for ATP production. For that, a semen straw from one bull (n = 3) previously tested for IVF was thawed and motile sperm were separated by Percoll gradient, washed and resuspended in FertTalp medium (FT) without capacitator agents (Parrish et al. 1989 Biol. Reprod. 41, 683–699) to a final concentration of 30 × 106cells mL−1. Then, samples were centrifuged and resuspended in 5 different groups: positive control (PC, FT supplemented with 2 mM glucose, 0.2 mM pyruvate, and 11 mM lactate); negative control (NC, FT without energy substrates); Glu (FT and 3.5 mM glucose); Pyr (FT and 0.11 mM pyruvate), and Lac (FT and 5.5 mM lactate). Samples were incubated at 38.5°C, 5% CO2 in high humidity for 15 and 45 min. After incubation, samples were centrifuged and supernatant was collected and analysed by Raman spectroscopy as previously described (Santos et al. 2015 Biomed. Opt. Express 6, 2830–2839; https://doi.org/10.1364/BOE.6.002830). Data were preprocessed and submitted to principal component (PCA) and loading plot analysis (LP) by using Minitab software (Minitab Ltd.). The results showed that after 15 min of incubation, the metabolic profiles were similar for the PC, Glu, and Lac groups, suggesting that they present similar metabolic activity. NC and Pyr were a separate cluster, indicating that pyruvate is not metabolized through OXPHOS in this phase. LP analysis comparing Glu and Pyr groups indicated phosphatidylserine, phenylalanine, DNA/RNA, and lipids as the most distinct metabolites. After 45 min, PC and Pyr had a similar metabolome profile, whereas NC, Glu, and Lac clustered together, suggesting that for long-period incubation OXPHOS takes place as the preferential pathway for energy production in bovine sperm cells. At this time, the comparison of Glu versus Pyr revealed phosphatidylserine, proline, phenylalanine, carboxylic acid, DNA/RNA, proteins, and lipids as the most different metabolites between groups. Based on these results, we hypothesised that the glycolysis to OXPHOS transition may be a consequence of the depletion of glycolytic enzymes, leading the sperm cells to use distinct pathways for long-term maintenance of ATP production. In conclusion, our data showed that the metabolome profile of bovine spermatozoa varies according to the period of incubation and substrates availability for energy production. However, more studies are necessary to characterise the ability of these metabolites to maintain sperm motility and viability. This research was funded by FAPESP 2017/18384-0.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,001 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».