The role of the V‐type H <sup>+</sup> ‐ATPase in CAPA‐treated Malpighian tubules in the yellow fever mosquito, <i>Aedes aegypti</i>
Notice bibliographique
Résumé
Haematophagus insects, such as the female Aedes aegypti mosquito, face the challenge of excess ion and water intake after engorgement on a blood meal. To cope with this, adult female A. aegypti utilize their highly adapted excretory system that includes the Malpighian ‘renal’ tubules (MTs), which are under rigorous control by several hormones to regulate transepithelial movement of ions/water. Originating in the nervous system, the mosquito anti‐diuretic hormone is a member of the CAPA peptide family and is a homolog of the vertebrate neuromedin U peptide. CAPA peptides inhibit fluid secretion of MTs stimulated by select diuretic factors, 5HT and DH 31 through the NOS/cGMP/PKG pathway. However, the anti‐diuretic signalling mechanism and downstream cellular targets, such as ion channels and transporters, remain unclear. Active ion transport in A. aegypti MTs is driven by the principal cells, whose brush‐border membrane is densely packed by the V‐type H + ‐ATPase (VA), serving as the primary energizer for transepithelial secretion of electrolytes and water. Due to its predominant role in fluid secretion, the VA is a likely target for diuretic and anti‐diuretic factors. The effect of endocrine factors and the VA inhibitor, bafilomycin, on fluid secretion rate by MTs was measured using an in vitro bioassay. Bafilomycin was found to inhibit fluid secretion stimulated by serotonin (5HT) and the calcitonin‐related diuretic hormone (DH 31 ), while having no inhibitory action on MTs stimulated with the corticotropin‐releasing factor (CRF)‐related diuretic hormone. CAPA and bafilomycin treatment led to alkalization of the secreted fluid suggesting inhibition of the apical VA, which may lead to constrained entry of cations (Na + and K + ) across the apical membrane of MTs. VA activity was increased in adult female MTs treated with DH 31 , whereas CAPA treatment reduced VA activity, comparable to saline levels. Furthermore, a VA antibody was used to determine protein localization and expression in DH 31 and CAPA‐treated MTs. In DH 31 ‐incubated MTs, the VA was exclusively localized to the apical memrane of the MTs, whereas VA immunoreactivity was observed in both the apical membrane and cytosol of CAPA‐treated tubules. These results suggest a novel mechanism for CAPA inhibition, blocking the VA to hinder fluid secretion. A. aegypti mosquitoes are vectors of a variety of pathogens leading to illnesses, such as Dengue fever, Yellow fever, and the Zika virus. The current results advance our knowledge of the anti‐diuretic control of the A. aegypti MTs, suggesting that CAPA targets the VA to inhibit diuresis. Investigating the pathway of CAPA inhibition and its role in countering diuresis will help provide a deeper understanding of this critical physiological process. Studying the excretory system and discerning the underlying mechanisms behind ion and fluid transport may aid in developing novel pest management strategies to better control Aedes population and lessen its prominence as a disease vector.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,002 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».