Cellular Photoprotective and Antioxidant Properties of Lipids in an Oral Skincare Supplement
Notice bibliographique
Résumé
Background Overexposure to ultraviolet (UV) light is associated with multiple health risks from sunburn to skin cancers. The ingestion of natural product photoprotectors can increase skin's UV‐resistance. Our research focuses on the cellular effects of Bend Beauty, Inc.'s oral supplement called “Bend Skincare Anti‐Aging Formula” (AAF), which contains daily doses of the ω‐3 fatty acids eicosapentaenoic acid (EPA; 1050 mg) and docosahexaenoic acid (DHA; 350 mg), ω‐6 fatty acid gamma‐linolenic acid (GLA; 120 mg), carotenoids zeaxanthin (2.5 mg) and lutein (5 mg), and vitamin D3 (1,000 IU). A clinical trial found that ingestion of AAF increased the minimal erythema doses (UV exposure required to induce skin redness) of volunteers' skin by 84% after 8 weeks, warranting further research into AAF's photoprotective properties. Objective To quantify AAF's photoprotective and antioxidant properties in human dermal fibroblasts. Methods & Results Experiments were completed in primary human dermal fibroblasts (passage #3) treated with 0.005% AAF or vehicle control (10% H 2 O, 10% THF, 75% DMSO, 5% FBS). For the initial photoprotection assays, cells were treated with AAF or vehicle for 1, 7, or 14 days and exposed to UVA (365 nm; 216 J/cm 2 ) or UVB (312 nm; 15.6 – 8,000 J/cm 2 ). The viability of 1, 7, and 14‐day AAF‐treated cells after UVA exposure was 2.9, 4.2, and 3.5‐fold higher, respectively, vs. control (P < 0.05), and the UVB IC 50s were 2.6 and 3.2‐fold higher in 7 and 14‐day AAF‐treated cells, respectively, vs. control (P < 0.05) as measured with MTT assays. For morphological analysis, 14‐day AAF‐ or vehicle‐treated fibroblasts were exposed to UVA (100 J/cm 2 ) or UVB (1 J/cm 2 ), stained with propidium iodide (PI; cell death marker), phalloidin (cytoskeleton stain), and Hoechst 32258 (nucleic stain) and imaged using confocal microscopy. After UVA or UVB exposure, the vehicle‐treated cells were visibly more damaged than AAF‐treated cells as assessed by actin staining, and PI staining was present in > 97% of the vehicle‐treated vs. < 8% of AAF‐treated fibroblasts (Figure 1). To quantify AAF's antioxidant effects, MTT assays were completed in 1, 7, and 14‐day AAF‐treated fibroblasts after 48 hours of treatment with the reactive oxygen species (ROS), H 2 O 2 (11.1 – 2,700 mM); H 2 O 2 's IC 50 values were calculated to be 2.0, 2.4, and 2.5‐fold higher, respectively, vs. control (P < 0.05). In identically AAF‐treated fibroblasts, assays to measure the intracellular ROS activity induced by H 2 O 2 (0.9 mM, 1 h) using a ROS‐fluorescent probe (CM‐DCFH 2 ‐DA) were completed. H 2 O‐ 2 ‐induced ROS activity was 67%, 63%, and 62% as high in 1, 7, and 14‐day AAF‐treated cells vs. control, respectively (P < 0.05). Conclusions AAF helps to protect human dermal fibroblasts from the damaging effects of UVA, UVB, and H 2 O 2 , demonstrating its cellular photoprotective and antioxidant properties. Support or Funding Information Project funding provided by Bend Beauty, Inc., Mitacs Elevate, and the Dalhousie Pharmacy Endowment Fund This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».