Abstract 3091: Relevance of iNOS expression in bladder cancer stem cells
Notice bibliographique
Résumé
Abstract Background: Bladder cancer (BC) is one of the most common tumors of the male urogenital tract and an important worldwide cause of death. Increasing evidence has indicated the presence of cancer stem cells (CSCs) in many types of tumors, associated with aggressiveness, chemoresistance and relapse. The expression of inducible nitric oxide (NO) synthase (iNOS) in human BC is a poor prognostic factor associated with increased invasion and tumor recurrence. Although there is evidence supporting the role of NO/iNOS in the promotion and progression of BC, the possibility that there are CSCs dependent on endogenous NO generation was not yet been clearly defined. In this study we evaluate the role of NO in CSC maintenance, modulating its production, using pharmacological inhibitors or silencing iNOS expression in a murine BC model. Also, we analyzed the correlation between iNOS expression and stemness-related genes (SG) in human BC samples with different invasion grades. Methods: iNOS pharmacological inhibitors (pan-NOS or iNOS specific) or shRNA were used on murine BC model with different iNOS expression and invasiveness grade: MB49 [iNOS+, non-muscle invasive (NMI)] and MB49-I [iNOS++, muscle invasive (MI)], in order to analyze cell proliferation, number of CSC determined as spheres forming efficiency (SFE) and SG (Sox 2, Oct4 and Nanog expression) by qPCR. iNOS expression and SG were also analyzed in human BC samples (16 NMI low grade (LG), 10 NMI high grade (HG) and 17 MI). Results: The number of CSCs was higher in MB49-I than NMI MB49 line (p<0.05), in concordance with the higher expression of iNOS and Sox2, Oct4 and Nanog (p<0.001). iNOS inhibition diminished CSC in both cell lines (p<0.05 vs MB49; p<0.001 vs MB49-I). Inhibition of SG was only observed in MB49-I line (p<0.05). Moreover, the reduction of CSC and SG were more evident in MB49-I-shiNOS (p<0.0001 vs MB49-I scr; p<0.001 vs MB49-I-scr). The in vivo tumorigenic potential of cells derived from MB49-I spheres were higher than the cells growing in monolayers. MB49-I spheres cells treated with iNOS inhibitors did not developed tumors in vivo. Furthermore, MB49-I shiNOS spheres showed an incipient growth and regressed about 5 weeks post inoculation, in correlation with low iNOS and SG expression. Regarding human samples, iNOS was expressed in 78% of MI, 55% HG and 50% LGNMI (p<0.05 vs NMI). SG expression were significantly higher in MI than in NMI LG. Sox2 was expressed 82% in MI, 25% LG and 40% HG in NMI (p=0.0002). 76% of MI samples were positive for Nanog and Oct4 (p<0.05 vs NMI LG). A positive correlation between iNOS and SG expression was found (sox2: p<0.0001; Oct4: p<0.0001 and Nanog: p<0.05). Conclusion: Together, this results show that iNOS plays an important role in maintenance of CSC and in vivo BC growth by regulating stemness and self-renewal ability. Thus, iNOS identification and inhibition could be a potential target to eradicate CSC, responsible for tumor recurrences. Citation Format: Denise Belgorosky, Yanina Langle, Julie Girouard, Jovane Hamelin-Morrissette, Lina Marino, Eduardo I. Agüero, Héctor Malagrino, Carlos Reyes-Moreno, Ana M. Eiján. Relevance of iNOS expression in bladder cancer stem cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 3091.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,002 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».