Abstract 1779: Dietary supplementation of haskap berry anthocyanin and probiotics attenuate the severity of DSS-induced acute colitis in Balb/c mice
Notice bibliographique
Résumé
Abstract Introduction: Colitis-associated colorectal cancer is a specific type of colorectal cancer that develops from long-standing chronic inflammatory conditions in colorectal epithelium. Genotoxic compounds produced by inflammatory cells can damage DNA and thereby can initiate malignant transformation. Haskap (Lonicera caerulea L.) berry, is a rich source of dietary anthocyanins, specifically cyanidin-3-O-glucoside (C3G). C3G has been shown to possess anti-inflammatory and anti-cancer properties. This study aimed at investigating the oral supplementation of free- or microencapsulated-haskap anthocyanin (310 mg/kg of body weight/mouse) alone or with probiotics (1×109 CFU/day) on the severity reduction of dextran sulphate sodium (DSS)- induced acute colitis in Balb/c mice. Methodology: The study was carried out in two main phases: 1) optimization of microencapsulation of haskap anthocyanin using novel coating materials; and 2) Investigating the combined effect of anthocyanin and probiotics on DSS-induced acute colitis in vivo. Maltodextrin and inulin (3:1 w/w) were used as coating materials and four different core to shell ratios (1:1, 1:1.5, 1:2 and 1:3) were tested. The best ratio (1:1.5) was selected based on the physical characteristics, encapsulation yield, efficiency, recovery, and anthocyanin retention in the particles and was then used in DSS induced acute colitis model. Thirty-five Balb/C male mice of seven weeks old were divided into seven dietary supplementation groups (n=5) to receive either free anthocyanin, microencapsulated anthocyanin, or probiotics alone or as combinations of anthocyanin and probiotics. The colonic inflammation of mice was induced by oral administration of DSS at 3% for 7 days. Attenuation of acute colitis was investigated through clinical data, western blot, histopathology, and ELISA. Results: None of the dietary supplements showed hepatotoxicity in experimental mice hence the tested amounts were in safe limits. Compared with the DSS alone group, anthocyanin + probiotics supplemented group significantly inhibited the severity of colitis as measured by colon shortening, and disease activity index score. All the supplementary diets reduced the expression of occludin but had no effect on claudin-3 and claudin-4 expressions. Histological assessments, measures of inflammatory mediators and characterization of the gut microbiome will be presented. Conclusions: Dietary supplementation of haskap berry anthocyanin + probiotics protects against DSS-induced colitis possibly through attenuating the epithelial inflammation. Citation Format: K.V. Surangi Dharmawansa, Andrew Stadnyk, H.P. Vasantha Rupasinghe. Dietary supplementation of haskap berry anthocyanin and probiotics attenuate the severity of DSS-induced acute colitis in Balb/c mice [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 1779.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».