Author Correction: Cell spinpods are a simple inexpensive suspension culture device to deliver fluid shear stress to renal proximal tubular cells
Notice bibliographique
Résumé
The original version of this Article contained errors in the key for Figures 7, 8 and 9 where the solid red square “rotating” was incorrectly given as “static”, and the striped square “static” was incorrectly given as “rotating”. The original Figures 7 , 8 and 9 and accompanying legends appear below. Figure 7 Effect of rotation in cell spinpods on the activity of xenobiotic efflux transporters. RPTEC/TERT1 cells on Cytodex carrier beads were cultured in cell spinpods under rotating ( ) or static conditions ( ) for 48 h and harvested. One aliquot of cells was chilled and cold CMFDA was added before immediate analysis to measure non-specific binding. The remaining cells were incubated with CMFDA for 40 min at 37 °C, washed, and the CMFDA/GM-SF was allowed to efflux in the presence and absence of the inhibitor MK571. The quantity of CMFDA/GS-MF in the cells was measured by flow cytometry immediately after washing (0 min efflux) and after 30 min of efflux at 37 °C (30 min efflux and 30 min efflux with MK 571). Asterisks indicate where differences in the distributions between groups were statistically significant, e.g. p < 0.05, by one-tailed Mann–Whitney U test. Non-specific binding was significantly higher in cells from rotating cell spinpods ( p = 0.00015, W = 2, n 1 = n 2 = 5). CMFDA/GS-MF remaining in cells from rotating cell spinpods was significantly lower at time zero ( W = 4, n 1 = n 2 = 6, p = 0.013), after 30 min of efflux ( p = 0.00035, W = 0, n 1 = n 2 = 5), and after 30 min of efflux in the presence of MK571 ( p = 0.065, W = 6, n 1 = n 2 = 6). The inset shows the difference in CMFDA/GM-SF signal between time 0 and after 30 min of efflux. The loss of CMFDA/GM-SF from cells in rotating cell spinpods was significantly larger ( p = 0.008, W = 24, n 1 = n 2 = 5). Full size image Figure 8 Effect of rotation in cell spinpods on effect of chemotherapeutic agents. RPTEC/hTERT cells on carrier beads were cultured in cell spinpods under rotating conditions ( ) or static conditions ( ) for two days in the presence of 5 µM doxorubicin, 100 µM cisplatin, or no drug control. Panel ( a ) shows the uptake of glucose, measured with the fluorescent substrate 2-NDBG. Panel ( b ) shows the uptake of FITC-albumin. Cells from all samples were trypsinized off the carrier beads before analysis by flow cytometry. Data is presented as mean fluorescence ± SEM of six replicates (except for 5 replicates with static cisplatin), in relative fluorescence units. Brackets mark where differences in the distributions between groups were statistically significant, e.g. p < 0.05, by one-tailed Mann–Whitney U test. Full size image Figure 9 Effect of rotation in cell spinpods on cytokine release from renal cells. RPTEC/TERT1 cells on Cytodex carrier beads were cultured in cell spinpods under rotating conditions ( ) or static conditions ( ) in the presence of myeloma light chains from donor B, myeloma light chains from donor C, or media. After 48 h, supernatants were harvested for assay of cytokines. Bars presented mean concentration (pg/mL) of GM-CSF, panel ( a ), IL-6, panel ( b ), and NGAL panel ( c ), in the cell supernatant; error bars are ± SEM. Asterisks indicate where differences in the distributions between rotating and static groups were statistically significant, e.g. p < 0.05, by one-tailed Mann–Whitney U test. Rotation induced significantly greater quantities of GM-CSF in the media control ( p = 0.004, W = 39, n 1 = 7, n 2 = 6) and after stimulation with myeloma light chains from donor C ( p = 0.005, W = 33, n 1 = 7, n 2 = 5). Rotation induced significantly greater quantities of IL-6 after culture in media alone ( p = 0.036, W = 34, n 1 = 7, n 2 = 6) and with myeloma light chains from donor B ( p = 0.001, W = 41, n 1 = 7, n 2 = 6). Rotation significantly reduced the quantities of NGAL released after stimulation with myeloma light chains from donor C ( p = 0.024, W = 5, n 1 = 7, n 2 = 5) and myeloma light chains from donor B (Fig. 8 c, p = 0.05, W = 9, n 1 = 7, n 2 = 5). Full size image
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,003 | 0,033 |
| Méta-épidémiologie (sens strict) | 0,002 | 0,001 |
| Méta-épidémiologie (sens large) | 0,002 | 0,001 |
| Bibliométrie | 0,003 | 0,002 |
| Études des sciences et des technologies | 0,003 | 0,002 |
| Communication savante | 0,003 | 0,002 |
| Science ouverte | 0,003 | 0,002 |
| Intégrité de la recherche | 0,007 | 0,013 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,024 | 0,022 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».