The Value Of A Specimen Tracking Tool And Beyond
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Notice bibliographique
Résumé
Background: It is generally recognized that the integrity of tissue specimens preserved at temperatures below the gas state is stable long-term. Studies in literature have not typically extended beyond a few years. As many biobanks today may store specimens for over a decade, this assumption should be tested to provide the data to support extended long-term storage and verify that stored samples continue to be fit-for-purpose. Since its inception in 2004, the Ontario Tumour Bank (OTB) has had an ongoing commitment to quality and, in accordance with biobanking best practices, has embedded stringent quality control (QC) and quality assurance (QA) measures into its routine procedures. One such measure, triggered twice annually, includes the random selection of cryopreserved tissues to undergo quality assessment (EXTQA) to measure the integrity of the tissueu2019s DNA and RNA. As an extension of OTBu2019s routine EXTQA, we analyzed second aliquots of previously evaluated tissues collected between 2005 and 2014 to determine if RNA (presented at the ISBER annual conference in 2017) or DNA integrity (presented here) is affected by extended long-term storage in liquid nitrogen vapour phase. Methods: As previously presented, RNA was extracted from duplicate aliquots of 70 cryopreserved tissue samples across 11 disease sites and quality was determined by the RNA Integrity Number (RIN) assigned by the Agilent Bioanalyzer. As an extended fit-for-purpose assessment, DNA was extracted from 20 samples to determine if DNA Integrity is associated with time in storage. The DNA integrity of the sample, as defined by its DIN value, was determined using the Agilent 2200 Tapestation and Genomic DNA Screen Tape.Results: As presented previously, there was no significant correlation between the quality of RNA versus storage time. New to this study, there was also no significant correlation between the quality of DNA versus storage time (r=0.250, p= 0.287). As a secondary observation, DNA quality is not correlated to RNA quality (r=0.2362, p=0.3147). Conclusions: This data suggests that, as for RNA, the extended long-term storage of tumour tissue samples in vapour phase in liquid nitrogen tanks does not negatively affect the quality of DNA derivatives. As a secondary observation, RNA integrity (RIN) is not a good predictor of DNA integrity, which supports previous recommendations to consider appropriate fit-for-purpose tests for different downstream applications rather than relying solely on RIN.
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Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,001 | 0,002 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découle