Prolonged survival of E coli but not <i>Staphylococcus aureus</i> in monocytes from patients with Crohn's disease
Notice bibliographique
Résumé
Introduction A primary macrophage defect has been proposed to play a role in Crohn's disease (CD) pathogenesis.1 In CD, Escherichia coli persist within lamina propria macrophages and peripheral blood monocytes are unable to effectively kill E coli in vitro.2 3 It is uncertain whether this abnormal bacterial handling by monocytes and macrophages in CD is limited to certain strains of E coli or whether a broader bacterial killing defect exists. The aim of this study was to compare intracellular survival of E coli with Staphylococcus aureus (not a usual resident of gut flora) within CD-derived and control monocytes. Methods Peripheral blood was taken from eight healthy controls and nine CD patients. CD distribution was ileal (n=1) and ileocolonic (n=8). Three CD patients were on thiopurines. Monocyte isolation was performed by iodixanol barrier flotation. Monocytes were challenged with a CD-derived strain of E coli and methicillin sensitive Staphylococcus aureus (MSSA). After 1 h incubation, the gentamicin protection assay was performed. Monocytes were lysed to release internalized bacteria after further incubation for 1 and 4 h. Cell lysates were plated on agar and incubated for 24 h at 37°C. Colony forming units (CFU) were counted after both 1 and 4h incubation. The CFU counts at these time points allowed relative replication to be determined. Results Viable CD-derived E coli were cultured from cell lysates at 1 and 4 h from 9/9 CD patients, and in 3/8 HC's (mean increase CFUs from 1 to 4 h: CD +394%, HC −7%). Viable S aureus was cultured in cell lysates at 1 and 4 h respectively from 1/9 and 0/9 CD patients and 0/8 healthy controls at both time points. The difference in the number of CFU's present for each subject at 1 and 4 h was calculated and there was a statistically significant difference in mean rank of (CFU (4 h) – CFU (1 h)) between CD and HC's for E coli but not for staph aureus (Mann–Whitney U test). Conclusion Within CD-derived peripheral monocytes, intracellular survival is prolonged for E coli but not for S aureus , illustrating that impairment of killing by monocytes in CD is restricted to particular bacteria. This is consistent with the clinical observation that CD primarily affects the gut which has an extensive but specific microbiome, rather than causing systemic immunodeficiency characterised by infections in other systems.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».