200 BONE MARROW MESENCHYMAL STROMAL CELL THERAPY FOR RESTORATION OF BLADDER WALL DEFECTS
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Résumé
You have accessJournal of UrologyStem Cell Research1 Apr 2012200 BONE MARROW MESENCHYMAL STROMAL CELL THERAPY FOR RESTORATION OF BLADDER WALL DEFECTS Jacques Corcos, Wally Mahfouz, Oleg Loutochin, and Jacques Galipeau Jacques CorcosJacques Corcos Montreal, Canada More articles by this author , Wally MahfouzWally Mahfouz Montreal, Canada More articles by this author , Oleg LoutochinOleg Loutochin Montreal, Canada More articles by this author , and Jacques GalipeauJacques Galipeau Atlanta, GA More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.253AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Bladder tissue engineering seems to be a promising avenue for tissue replacement in neurourology and oncology. Our aim is to evaluate the process of bladder regeneration after implantation of bladder acellular matrices (BAMs) seeded with bone marrow derived-mesenchymal stem cells (MSCs) in experimentally damaged bladders. METHODS 72 Sprague-Dawley rats were divided as follows: 6 for harvesting MSCs, 22 for harvesting bladders, and 44 subdivided into 8 subgroups: normal, sham control, partial cystectomy (PC), augmentation with BAMs only and augmentation with MSCs seeded BAMs. At 1 and 6 months, rats underwent cystometrograms before sacrifice, histological analysis and immunohistochemistry after sacrifice with H&E and Masson's trichrome. Urothelial cells were identified by anti-pancytokeratins AE1/AE3 while smooth muscle cells were identified by alpha smooth muscle actin (á-SMA). RESULTS PC group had only 45% of their native bladder capacity at 1 and 6 months. The non-seeded group increased their mean bladder capacities (MBCs) by 55% compared to pre-operative values at 1 month and 65% at 6 months. MSCs-seeded BAMs bladders reached MBCs of 75% and 99% in comparison to original pre-cystectomy volume at 1 and 6 months, respectively. In the MSC-seeded group, organized muscle bundles were present in the grafted patches at 1 month, yet their thickness was attenuated in comparison to normal control bladders. At 6 months, muscle bundles were more prominent with increased thickness. Strong and diffuse á-SMA expression was observed. In non-seeded BAMs, smooth muscles were identified, but in disorganized arrangements and attenuated thickness compared to seeded groups. Multi-layered urothelial regeneration was observed in all groups (anti-pancytokeratins positive). CONCLUSIONS Seeding MSCs onto BAMs is feasible and produce a viable tissue for bladder augmentation. Bladders grafted with MSCs-seeded BAMs showed a more significant increase in capacity and satisfactory muscularis propria development when compared to bladder augmented with BAMs only. Functionality of this new type of biomaterial is still to be proven. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e84 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Jacques Corcos Montreal, Canada More articles by this author Wally Mahfouz Montreal, Canada More articles by this author Oleg Loutochin Montreal, Canada More articles by this author Jacques Galipeau Atlanta, GA More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,001 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,038 | 0,006 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».