Mobile Water Kit 2.0: A Field Deployable Solution for E. coli Detection in Potable Water
Notice bibliographique
Résumé
Escherichia coli (E.coli) is an indicator organism, the presence of which in potable water suggests that the water source has been compromised. Consumption of such water would cause huge health problem and even human mortality. However, one of the key challenges for water quality monitoring is to detect such indicator organism right at the source or at the point of consumption. Current technologies rely on collecting water samples and sending samples to microbiological laboratories, which through membrane filtration and plate count, provide conclusive results, typically within 24 – 48 hrs. Such lengthy wait period is very much precarious to the communities, particularly in rural Canada, counties in US, and limited resource regions of developing economies (i.e., India, China, Brazil), where even access to a reliable water quality monitoring laboratory is questionable. In response to this global issue, we have developed a low-cost and extremely efficient test method known as the Mobile Water Kit (MWK) [Gunda et al., Analytical Methods , 6, (16), 6236-6246, 2014], which has greatly simplified the process of E.coli detection in the field, which wasn’t feasible until recently. The MWK can accurately detect extremely low concentrations of E.coli (2 CFU/100 mL) under an hour and is faster than the current conventional laboratory methods, which requires 24 to 48 hours to produce proper results. However, MWK may require a trained technician to perform the test as there are number of steps involved for water testing starting from collecting the water sample to broadcasting of test results. Moreover, MWK contains the chemical reagents in liquid form to be stored and handled in a careful and manageable way. In the present work, we resolved these two impediments by the following modifications to MWK: (a) reduced the number of steps required to perform water testing; (b) converted the liquid based system to a hydrogel impregnated chemical reagent based system (i.e., porous soft material) for ease of handling and deployment. This revised version of MWK (i.e., MWK 2.0) simplifies the testing method and make it amenable for regular field use without the help of a trained technician. In addition, the MWK 2.0 replaces syringes, filters, centrifuge tubes, pipette tips, etc., which were essential components for the original version of MWK. Moreover, MWK 2.0 allows sample concentration and detection in one single step. With MWK 2.0, we were able to detect E.coli concentrations of 4x10 6 CFU/mL to 4x10 5 CFU/mL within 5 min and 4x10 4 CFU/mL to 40 CFU/mL within 60 min.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».