6-Gingerol, but Not Whole Ginger Juice, Specifically Inhibits Growth of Colon Cancer Cells in Culture
Notice bibliographique
Résumé
Colon cancer is affluent among many people, and having cancer greatly impacts their lives. Ginger is a common food, particularly in Asian cuisine. However, the health benefits of ginger and 6-gingerol, as its bioactive component in the prevention of colon cancer, have not been fully addressed. This experiment investigated the effects of ginger juice and 6-gingerol on colon cancer cell growth and death. Colon cancer SW480 cells and CCD-18Co normal colon epithelial cells were purchased from ATCC.SW480 cells were grown in DMEM with 4.5 g/L glucose supplemented with 10% fetal bovine serum (FBS), penicillin, and streptomycin, and normal colon CCD-18Co cells were maintained in EMEM with 4.5 g/L glucose and glutamine with 10% FBS. 6-gingerol was dissolved in DMSO in a stock of 100 mmol/L. Ginger roots were homogenized and the juice was collected through 3 layers of cheesecloth filtering, followed by centrifugation and 0.2 μm filter sterilization. About 5000 cells were seeded in a 24-well plate and treated with various amounts of ginger juice and/or 6-gingerol for up to 72 hours. Cell growth was examined using Trypan blue stain, and cell cycle arrest was determined by immunoblotting using antibodies against key proteins in cell cycles. Data were analyzed by two-way ANOVA with a Tukey posthoc test and statistical significance was set at P < 0.05. Time course and dosage curve experiments showed that 6-gingerol significantly inhibited SW480 cell numbers starting at 0.5 μM (P < 0.005). More than 1 μM 6-gingerol did not give more power to inhibit SW480 cell growth. The results also showed that CCD-18Co cell numbers were not changed after 6-gingerol treatments (P > 0.1). Low dosages of ginger juice (2,000 x- to 100 x- fold dilutions) didn’t affect the growth of both cell types. Immunoblotting results revealed that the elevation of pSer10-CDC2 levels and decreases in p21 Wafl/Cip1 and pSer642-Wee1 only occurred in SW480 but not CCD-18Co cells when treated with 1 μM 6-gingerol for 40 hrs. Through this experiment, it can be concluded that 6-gingerol can kill SW480 cancer cells without killing normal CCD-18Co cells through cell cycle arrest. Further experiments could be run to discover more properties of 6-gingerol, how it works, and how it could be used in the medical world. Oklahoma State University internal funds to support high school student research.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,001 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,002 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».