Introduction of Session 8, “Structural mechanism of animal rhodopsins and GPCR”
Notice bibliographique
Résumé
SudoG protein-coupled receptors (GPCRs), seven helical transmembrane proteins, are responsible for signal transduction pathways by detecting diffusible signaling molecules such as hormones, neurotransmitters and odorants.Animal rhodopsins are highly specialized photoreceptor GPCRs for vision and function via isomerization by the light absorption of retinal chromophore (as a signaling molecule).The dynamic structural information of rhodopsin and GPCRs has recently been elucidated through several types of spectroscopies and structural analyses.In the session 8 chaired by me and Dr. Gebhard F. X. Schertler from Paul Scherrer Institute, five researchers presented cutting-edge results regarding 'structural mechanism of rhodopsins and GPCRs', as described below.The first speaker was Dr. Hideaki Kato from the University of Tokyo, who discussed the 'structural and functional diversity in pump-like cation channelrhodopsins'.To optically control cell excitability, optogenetics has revolutionized neuroscience.To broaden the application of optogenetics, increasing numbers of ion-translocating rhodopsins (ion pumptype rhodopsins and ion channel-type rhodopsins) have been engineered or discovered in nature.Recently, a new type of channelrhodopsins, pump-like cation-conducting channelrhodopsins or bacteriorhodopsin-like channelrhodopsins (PLCRs or BCCRs), have been discovered and attracted broad attention due to their unique properties (e.g., large photocurrents, high light sensitivity, high ion selectivity).In his talk, he presented the cryo-EM structures of PLCRs [1] and discussed their structure-function relationships.The next speaker was Dr. Oliver P. Ernst from University of Toronto, who discussed 'activation studies of opsin and adenosine A2a receptor'.In his talk, he presented studies on the activation of the apoprotein opsin by odorants.Three crystal structures of the active conformation of opsin, Ops*, with monoterpene odorants in the orthosteric retinal binding pocket, as well as biochemical, biophysical and electron paramagnetic resonance (EPR) [2] studies reveal how these odorants can act as agonists to promote conformational changes towards Ops*.He also presented the conformational equilibrium of adenosine A2a receptor.The third speaker was Dr. Xavier Deupi from Paul Scherrer Institute, who talked about 'structure and activation of lightactivated G protein-coupled receptors'.A wealth of biophysical, functional, and structural data have made the mammalian visual low-light receptor rhodopsin a paradigm of the GPCR family.Recently, they discovered that such similarities are not limited to vertebrate rhodopsins but extend even to visual receptors from invertebrates [3].In his talk, he presented (i) a novel position for the retinal counterion in the non-bistable box jellyfish rhodopsin that is discovered by employing computational structural models validated by mutagenesis and activity assays, and (ii) the structure of an early active intermediate of bovine rhodopsin (obtained at the SwissFEL free-electron laser) suggesting that the earliest structural rearrangements upon activation already appear in regions involved in later stages of the conserved class A GPCR activation mechanism.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».