Effects of omega-3 supplementation on markers of autophagy, apoptosis and mitochondrial area density
Notice bibliographique
Résumé
In humans, disuse is known to result in muscle atrophy and metabolic dysfunction, including reduced mitochondrial content. Previous data from this cohort found that omega-3 polyunsaturated fatty acid (n-3) supplementation attenuated declines in muscle mass, myofibrillar protein synthesis and ADP-stimulated mitochondrial respiration. Disuse has also been shown to increase apoptosis and events linked to autophagy. We investigated the effects of n-3 fatty acid supplementation on content of proteins involved in autophagy, intrinsic apoptosis, mitochondrial fission and fusion and mitochondrial area density during 14 d of immobilization and 14 d of recovery. Twenty females (18-30y) received 20mL doses of n-3 fatty acid (2.97g EPA and 2.03g DHA) or an isoenergetic sunflower oil (oleic acid content: 75%) daily during the 28-d protocol. Biopsies were taken from the vastus lateralis pre-immobilization, post-immobilization, and post-recovery. Protein and mitochondrial content were determined using Western Blot and transmission electron microscopy at 5800X magnification, respectively. Mitochondrial area density (%AD) decreased in the subsarcolemmal (SS, p=0.006) and central intramyofibrillar (IMF) (p=0.001) regions after recovery. The change in the central IMF was driven by a decrease in the n-3 group (p=0.0133). There was no change in the peripheral IMF region. P62 increased during immobilization (p<0.0001) and decreased in recovery (p<0.0001). After immobilization LC3B-I increased in the n-3 group only (p=0.0289). Procaspase-3 content was higher in the n-3 group at all timepoints, D0 (p<0.001), D14 (p<0.0001), D28 (p<0.0001). Procaspase-3 increased after immobilization in the placebo group (p=0.01), and after recovery in the n-3 group (p=0.00807). Procaspase-9 increased during immobilization (p=0.02) and remained elevated through recovery (p=0.00593), with a notable increase in the n-3 group after recovery(p=0.00923). BAX increased in recovery in the n-3 group (p=0.00137) whereas it increased during immobilisation in the placebo group (p=0.02). BCL2 decreased post-recovery (p=0.00671) and the ratio of BCL2:BAX decreased during immobilization (p<0.001) and remained low through recovery (p<0.0001) in the placebo group, whereas the BCL2:BAX ratio decreased post-recovery in the n-3 group (p=0.00712). There was no effect of immobilization or supplementation on OPA1 or FIS1. Our findings show that mitochondrial %AD did not change during immobilization, but decreased in recovery specifically in the SS and central IMF regions of the myocyte. Furthermore, we found that immobilization increased markers of apoptosis and autophagy, but not mitochondrial fusion or fission, and that supplementation attenuated these changes until after recovery. This highlights the importance of considering recovery periods after immobilization and adds to our understanding of how immobilization and n-3 supplementation influence muscle metabolic health. This study was supported with a Canadian Institutes of Health Research Award to S.M. Phillips and a Natural Sciences and Engineering Research Council of Canada Award to M.C Devries This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.
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Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».