FRI386 Effects Of Maternal Sucrose Intake During Pregnancy On Steroids In The Placenta And Fetal Brain Of Rats
Notice bibliographique
Résumé
Abstract Disclosure: M.M. Jung: None. D.R. Seib: None. H.W. Chen: None. M. Idrissi: None. K.K. Soma: None. Intake of added sugars such as sucrose (table sugar) is high globally. Many people consume more than 20% of their daily calories as added sugars. High-sucrose consumption alters steroids and the brain. In rats, a human-relevant level of maternal sucrose intake starting 10 weeks before, during, and after pregnancy decreases maternal corticosterone in the serum but increases corticosterone in the blood and brain of the adult female offspring. Similarly, rat dams fed a high-sucrose diet starting 10 weeks before and during gestation have elevated 11-dehydrocorticosterone (DHC) in the serum and amniotic fluid and reduced testosterone and androstenedione in the labyrinth/junctional zone and decidua, respectively. Aldosterone is also elevated in the amniotic fluid, fetal blood, and fetal brain (nucleus accumbens, NAc). Thus, it is clear that maternal sucrose intake alters both maternal and offspring steroids, and has long-term, sex-specific endocrine effects. However, it is unclear how maternal sucrose consumption specifically during pregnancy impacts the offspring development. Our study investigates how maternal sucrose intake during pregnancy affects steroids in the placenta and fetal brain. We hypothesize that maternal sucrose intake is a metabolic stressor that will alter steroid signalling in the placenta and the developing brain. We fed female Long-Evans rats either a high-sucrose diet (26% kcal sucrose) or an isocaloric, macro/micro-nutrient-matched control diet (1% kcal sucrose) starting embryonic day 0 (E0; the day of pregnancy confirmation) (n=15/group). On E19, we collected maternal serum, amniotic fluid, placenta, fetal blood, and fetal brain. We will microdissect the placenta (decidua and labyrinth/junctional zone) and fetal brain (prefrontal cortex, NAc, ventral tegmental area, hippocampus, and hypothalamus) and measure a panel of 15 steroids using highly specific and sensitive liquid chromatography-tandem mass spectrometry. Maternal sucrose intake did not alter maternal food intake, body weight, and litter size, but increased the percentage of male offspring. We are currently analyzing the steroid data. We predict that maternal sucrose intake during pregnancy will increase anti-inflammatory steroids (corticosterone, DHC, and aldosterone) in the maternal blood, amniotic fluid and fetal brain, and decrease androgens in the placenta. We will also measure steroidogenic enzymes in the placenta and fetal brain. Our findings will clarify how maternal sucrose intake impacts both the placenta and fetal development. Moreover, our results will examine whether sucrose exposure during pregnancy is the underlying mechanism for the endocrine effects observed in the fetal and adult offspring. This study will provide more insight into the relationship between nutrition, the nervous system, and the endocrine system. Presentation: Friday, June 16, 2023
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Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
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