912 A comparison of 37+ immune populations in whole blood by CyTOF® flow cytometry collected with 4 different preservation reagents
Notice bibliographique
Résumé
<h3>Background</h3> Immune profiling studies using whole blood (WB) specimens are increasingly common in research and clinical research settings. It is crucial to standardize sample collection, shipping, and downstream processing of specimens for large studies. WB preservation reagents have been developed to address these logistics challenges. Using a lyophilized CyTOF® 30-marker immune profiling panel, this study aims to assess the effect of Streck Cyto-Chex®, BD PAXgene® Blood DNA Tube, Smart Tube Proteomic Stabilizer PROT1, and Cytodelics preservatives on data quality. CyTOF® flow cytometry allows for simultaneous detection of 50-plus parameters in a single tube without the need for single-stain controls. CyTOF uses typical antibody staining protocols and overcomes spillover and autofluorescence by using antibodies conjugated to heavy metal isotopes. <h3>Methods</h3> We compared 37+ immune populations in WB drawn from three healthy donors into BD heparin, Cyto-Chex, and PAXgene blood collection tubes. As a baseline, blood from the BD heparin donors was stained with the Maxpar® Direct® Immune Profiling Assay kit and T Cell Panel 3 with <24h post-blood draw. For Cytodelics stabilizer and Prot1, aliquots from BD heparin donor tubes was combined with stabilizer and frozen <24h post-blood draw. Remaining blood from the BD heparin, Cyto-Chex, and PAXgene tubes was stored at room temperature (RT). At 48h post-draw, blood stabilized in Cytodelics stabilizer and PROT1 was thawed and RBC lysed. All samples were stained following the Maxpar Direct Immune Profiling Assay protocol. Samples were acquired on the CyTOF XT™. Data was analyzed manually and by automated analysis using Maxpar Pathsetter™ software. <h3>Results</h3> Compared to the baseline at 24h, the frequencies of most populations remained similar across conditions however, the median staining intensity of most markers was reduced for all conditions. Major immune cell populations were identified when WB was stored at RT in Cyto-Chex and PAXgene tubes. Major populations were also preserved in PROT1 and Cytodelics, however, the expression of chemokine receptors was altered. <h3>Conclusions</h3> This study provides a comparison of WB preservation methods and their compatibility with a lyophilized CyTOF immune profiling panel. All stabilizers allowed major immune populations to be identified at 48h post thaw, although signal intensities of markers were reduced. An appropriate preservation method combined with lyophilized CyTOF antibody panels enables deep immune profiling when access to instruments, expertise and sample volume is limited in large/multi-site studies. <h3>Ethics Approval</h3> The samples obtained for this study were sourced from an accredited commercial provider.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».