Assessing myocardial microvascular reactivity with a novel MRI imaging approach as an early biomarker of diabetic heart failure
Notice bibliographique
Résumé
Abstract Introduction Microvascular dysfunction (mvD), and more specifically coronary microvascular disease (CMD), has been implicated as the primary hallmark of diabetic cardiomyopathy and HFpEF, afflicting millions of people worldwide. The onset and progression of microvascular disease is driven by vascular inflammation and is characterized by vascular smooth muscle cell thickening and impaired vasomodulation, both of which ultimately reduce perfusion and damage tissue. To meet metabolic demands, the body recruits additional assistance from our microvascular reserve, which is diminished during stress, thus resulting in a dampened vasomodulatory response to stimuli. Diagnosing mvD in the myocardium, therefore, requires a non-invasive method to measure vasomodulation or, more precisely, changes in microvascular blood volume. Purpose Current clinical imaging platforms lack the ability to detect vasomodulation in a sensitive and specific manner. Because of this shortcoming, there is also no literature sex comparison of cardiac microvascular reactivity in response to stress in diabetics. This work aims to address this technology and knowledge gap by developing and demonstrating a novel MRI technique for the specific assessment of vasomodulation without confounding influences from changes in blood oxygen saturation, hematocrit, or flow. Methods Elevated CO2 is a safe and reliable vasodilatory stimulus and is effective in differentiating healthy from diseased vasculature. 10% CO2 was mixed with 21% oxygen and directed into an intubated rat. A blood-pool T1 contrast agent (Ablavar) was injected intravenously as a bolus (0.3mmol/kg) followed by a saline flush, to saturate the blood volume fraction, eliminating sensitivity to molecular oxygen and producing changes in T1 dominated by the blood volume fraction. The extended residency time of Ablavar, which stems from protein binding, allows a prolonged period of stable T1 signal enhancement (approx. 40 minutes). Results Exposure to 10% CO2, a known cardiac vasodilator, elicited conflicting results when compared across sexes. Young female rats demonstrated a strong vasodilatory response within 10 minutes of hypercapnic exposure, quantified through the drastic reduction in T1, while their male counterparts exhibited little to no change. When reverting to room air following 10 minutes of CO2, both male and female animals exhibited strong vasoconstriction. Young pre-diabetic female rats exhibited a blunted response when exposed to 10% CO2, losing their ability to vasodilate and constrict. Conclusion This work described a novel MRI diagnostic tool for highly specific assessment of microvascular vasomodulation and demonstrated a greater vasodilatory response to hypercapnic stimuli in healthy female rats compared to male, along with blunted response in diabetic females. This non-invasive technology will be valuable for early diagnosis of cardiac disease in patients predisposed to developing mvD.T1 myocardial microvascular reactivityBlunted pre-diabetic female stress-CMR
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,001 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,001 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».