β2‐1 Fructan Supplementation in Healthy Adults Elicits Host‐Specific Bacterial Responses But Does Not Support a Defined Health Benefit
Notice bibliographique
Résumé
Little data exists on physiological outcomes associated with bacterial changes that occur after fructan feeding in healthy adults. To this end, we studied potential relationships between changes in gut bacteria composition and host physiological parameters after feeding 3 × 5 g/d of β2‐1 fructan (BF) or maltodextrin to a group of 13 male and 17 female healthy adults in a placebo‐controlled, double‐blinded, randomised crossover‐study consisting of two 28‐d exposures separated by a 14‐d washout. Fasting blood and 1‐d faecal collections were obtained on d0 and d28 of each phase. Well‐being and general health, as well as gastrointestinal symptoms, were determined by questionnaire. Serum lipopolysaccharides (LPS), faecal SCFA, faecal bifidobacteria and indigestion were higher during the BF‐supplemented phase. Numbers of circulating lymphocytes and macrophages were unchanged but BF supplementation decreased serum IL‐10 and increased serum IL‐4. In addition, circulating percentages of CD282 + /TLR2 + myeloid dendritic cells increased as did ex vivo responses to a TLR2 agonist. Culture‐based analysis of feces showed that 87 bacterial species encompassing 30 genera and four phyla were able to use BF as the sole carbon source. Fecal 16S rRNA analysis (26 subjects) showed BF reduced community richness. Two response patterns were observed: in 17/26 subjects, the relative abundance of Bacteroides was reduced and phylotypes within the Bifidobacterium , Faecalibacterium and the family Lachnospiraceae increased during the BF phase. In the remaining subjects, phylotypes aligning within the genera Bacteroides, Prevotella and to a lesser extent Bifidobacterium increased in abundance while abundance of phylotypes within the Faecalibacterium and the family Lachnospiraceae decreased during the BF phase. Few relationships between the faecal community composition and measured parameters were noted: (a) increases in Bacteroidetes relative abundance correlated with increased faecal propionate; (b) subjects in whom Bacteroidetes increased during the BF phase excreted more caproic acid (during both phases); and (c) subjects in whom Bacteroidetes decreased during the BF phase had higher LPS and LPS binding protein (during both phases). The most likely explanation for the observed patterns of faecal community change associated with the BF phase is a distal gut fermentation driven by differences in peptidyl nitrogen. We found no links between bacterial community changes and physiological changes nor was there evidence of physiological changes that would indicate a health benefit in these healthy subjects. Support or Funding Information This trial was undertaken with financial support from Agriculture and Agri‐Food Canada (RPBI# 1501, MK, GDI, LJY), Health Canada (SB), General Mills (MK), Alberta Innovates Bio Solutions (GDI), the Advanced Food and Materials Network (MK), and the National Science and Engineering Research Council (JMJG).
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,001 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,001 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».