Portable CIP-based microfluidic platform for optical bacteriadetection
Notice bibliographique
Résumé
The increasing need, along with the advancements in microfluidics technology, has sparked the transition from conventional pathogen detection methods to faster, miniaturized, and cost-effective point-of-care (POC) and point-of-use (POU) technologies for use in remote and user settings.Microfluidic technology, commonly known as "Lab-on-a-Chip" (LOC), is a versatile, multipurpose instrument that amplifies scientific findings in its microchannels.On the other hand, as a steady and trustworthy indication in the bacteria detection system, Cell Imprinted Polymers (CIPs), synthesizes highly selective sorbents that are customized to our target bacteria.The integration of microfluidics and imprinted polymers has become a promising technique in biosensing applications in the recent years.We have previously developed a facile approach to coat bacterium-specific CIPs on florescent magnetic microparticles (MMPs) as a biosensing interface.CIP-coated MMPs (CIP-MMPs) were captured in a microfluidic device using external magnet and internal magnetic iron-PDMS microstructures, then fluorescent attenuation microscopy was used for proof-of-principle E. coli detection, achieving a limit of detection (LOD) of 10 2 CFU/mL.In this work, we introduce a portable optical platform that employs our MIP-based microfluidic biosensor to detect bacteria cells in water.The platform consists of portable optical and mechanical components that are assembled onto a 3D-printed housing.The system is powered by a portable laptop that also serve as the controller for the micropump and the display for the microscope.The micropump precisely controls the flow to the microfluidic chip while a fluorescent image of the detection area is displayed in realtime.To investigate the effect of bacteria concentration on capturing efficiency, E. coli OP50 solutions of different concentrations of 0, 10 3 , 10 5 and 10 7 CFU/mL were prepared in LB and run through the device.The CIP-MMPs at 0 concentration of bacteria were exhibiting a slight decrease in their fluorescent intensity over time, indicating a possible 10-15% decrease due to photobleaching.The fluorescent intensity reduction in 10 3 CFU/mL bacteria concentration was not significantly different compared to 0 concentration.The significant reduction in florescent intensity was observed in 10 5 and 10 7 CFU/mL concentrations of bacteria (48% and 65% respectively).This result confirmed the limit of detection (LOD) of ~10 5 CFU/mL for our designed sensing platform.Using this setting, we were able to develop a portable and low-cost platform technology for detection of bacteria at low LOD and wide concentration range which can be considered as an applicable water quality monitoring tool.
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Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».