Abstracts of the 8th Annual Conference of the Movement Disorders Society of India (MDSICON 2023-24)

1 Gut microbiome in Parkinson’s disease: Insights from a microglial model Avanteeka Ganguly, Joanna Korecka-Roet1, Vikram Khurana1, Hrishikesh Kumar Department of Neurology, Institute of Neurosciences, Kolkata, West Bengal, India, 1Department of Neurology, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA E-mail: [email protected] Background: Research has demonstrated that the gut microbiome can modulate neurochemistry and behaviors through the microbiota–gut–brain axis. Certain microbial species have been associated with specific neurologic diseases. Uncovering the mechanism by which the microbiota can modulate brain microenvironment is crucial to understand its role in disease pathogenesis. Objectives: We aimed to study the impact of gut microbiome on Parkinson’s disease (PD) in in vitro human stem cell–derived microglia models. Methods: Human microglial cells were cultured from induced pluripotent stem cells (iPSCs) using standard protocol. Microbiota were isolated from the fecal samples of PD patients, and genetic sequencing was done for the identification of bacterial flora. Selected bacterial species (based on past literature and documented association with PD and neurodegenerative diseases) were isolated in pure culture. The mature cultured microglia were then treated with supernatants from the bacterial cultures. They were then treated with fluorescent α-synuclein preformed fibrils (PPFs) and pHrodo for phagocytosis assay. Literature search was performed to select relevant microglial markers regulated by the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κβ) signaling cascade, and quantitative polymerase chain reaction was performed to study the expression of these markers by the treated microglia. Results: The microglia treated with bacterial supernatants and the positive controls showed an enhanced expression of the M1 markers (CCL2, CD-40, IL-1β, TLR-2, and TNF-α) with respect to PBS controls. This indicates a shift in microglial activation toward the M1 phenotype, and subsequently an inflammatory microenvironment in the brain, modulated by the presence of the characteristic microbiome. Interestingly, the expression of M2 markers, TGF-β and IL-10, also seemed to be higher in the positive controls and treated microglia than PBS controls. This could potentially signal toward a heterogeneous activation of the microglia in response to the stressors or could also be due to assay-related conditions. Imaging showed an active uptake of PFFs by the microglia. pHrodo treatment showed enhanced phagocytosis by microglia treated with lipopolysaccharide and bacterial supernatants, compared to PBS controls. Conclusions: All in all, we see an upregulation of the NF-κβ cascade, and increased phagocytosis leading to an inflammatory phenotype in the treated human microglial model of PD, and further experimentation can help elucidate the intricate relationship between the microbiome, neuroinflammation, and disease pathogenesis in greater detail. References 1. Huang Y, Wu J, Zhang H, Li Y, Wen L, Tan X, et al. The gut microbiome modulates the transformation of microglial subtypes. Mol Psychiatry 2023;28:1611-21. 2. Abud EM, Ramirez RN, Martinez ES, Healy LM, Nguyen CHH, Newman SA, et al. iPSC-derived human microglia-like cells to study neurological diseases. Neuron 2017;94:278-93.e9. Abstract 2 Altered salivary redox profile in Parkinson’s disease patients Swarnava Sengupta, Rebecca Banerjee, Jyoti Rungta, Sabbir Ansari, Supriyo Choudhury, Akash Roy, Hrishikesh Kumar Department of Neurology, Institute of Neurosciences Kolkata, Kolkata, West Bengal, India E-mail: [email protected] Background: Oxidative stress is central to the pathogenesis of Parkinson’s disease (PD). We found elevated oxidative stress in the peripheral blood of PD patients, which correlated with disease severity. Studies have highlighted the potential of saliva, a noninvasive tool for identification of disease biomarkers. Therefore, saliva from PD patients may be explored further for the assessment of disease oxidative burden. Objectives: Here, our aim was to ascertain the presence of particular oxidative stress determinants in the saliva of PD patients and explore their association, if any, with severity or phenotype of the disease. Methods: Forty PD patients and 30 age-matched healthy controls were recruited. Motor and cognitive severity assessments were performed using the Movement Disorders Society Unified Parkinson’s Disease Rating Scale (UPDRS III), Hoehn and Yahr, and Montreal Cognitive Assessment (MoCA) scores. PD patients were categorized into tremor dominant (TD) and postural instability and gait disorder (PIGD) phenotypes using UPDRS. Oxidative stress markers, namely, superoxide dismutase-1 (SOD-1) and catalase enzymes, as well as reduced glutathione (GSH) content were estimated by spectrophotometric methods. Correlations between clinical severity and oxidative stress indicators were investigated. Results: Salivary SOD-1 (P = 0.014) and catalase (P = 0.047) enzyme activities were found to be significantly lower in PD patients compared to healthy controls. GSH levels did not differ between the two groups. The salivary markers were not associated with UPDRS III, Hoehn and Yahr, and MoCA scores. No statistical difference was observed in the levels of oxidative stress markers between the PIGD and TD phenotypes. Conclusions: The activities of the primary antioxidants SOD-1 and catalase were reduced, indicating a higher degree of oxidative stress in the saliva of PD patients. The correlation of oxidative salivary markers with clinical disease severity among PD patients may be determined by conducting adequately powered longitudinal studies. References 1. Roy A, Mondal B, Banerjee R, Choudhury S, Chatterjee K, Dey S, et al. Do peripheral immune and neurotrophic markers correlate with severity of Parkinson’s 2. J, to tremor dominant and postural with the disorder Parkinson’s disease with the Parkinson’s disease Abstract in Parkinson’s disease Institute for Medical Research and of and of Research and of Parkinson’s Research Institute of and The of of The of and and of and of E-mail: [email protected] Background: Parkinson’s disease (PD) is a disease of heterogeneous The genetic in the and that associated with clinical genetic a of leading to is not genetic Objectives: The of the study was to which specific to PD genetic and which between the subtypes. we the and and to from the PD genetic the conditions. Methods: pluripotent stem cells were from peripheral blood cells or of PD patients in the and and from healthy controls. were for and for controls. The cells were to cells and then to mature and was to a of of of cells and their and Results: to and reduced healthy and reduced and in to controls and PD were the to induced from reduced healthy and reduced oxidative was specific to mature genetic showed to controls and and reduced and increased and reduced by that than may to in and PD Conclusions: This study that the genetic of PD differ in their PD increased that to and in genetic that a may be for PD patients on these Abstract the role of brain in in neurodegenerative Department of Neurology, Institute of Neurology, E-mail: [email protected] Background: by oxidative and in is in specific further to oxidative stress and is for in Objectives: We that a role in the of which subsequently and in as Parkinson’s and Methods: we a of in and in the brain to the the was from the of the of healthy controls and with using We the to the of using the were to have in disease compared to controls. Results: study of in the of that be associated with the in in further to associated that showed in in the and and to respect to the study by et al. on expression of we that we found in the and between the two which showed expression in with our Conclusions: We to the the role of in the of that to in This study can further in to understand the of the and help to to or the on the References 1. Y, J, Chatterjee A, et al. of and in 2. J, L, et al. of between the and of the disease. Abstract of and with Parkinson’s disease and in Parkinson’s disease of and Institute of Human India E-mail: [email protected] Background: Parkinson’s disease (PD) is the of neurodegenerative of the the of have and in the pathogenesis of Objectives: We aimed to study the association of and and with PD, with and and in We also to the with its of these to PD and healthy controls. Methods: of was performed in study PD and healthy controls to the association among biomarkers. was performed in PD and controls. Results: The of showed that were significantly higher (P levels of and in PD than controls. was performed to the that between the of an = PD and and a of and were into the to be the among the The was and in PD and the of and was and in Conclusions: we demonstrated the of and to PD from healthy controls. of the model in a is in studies. Abstract in Supriyo Choudhury, Rebecca Banerjee, Swarnava Sengupta, Sabbir Ansari, Hrishikesh Kumar Department of Neurology, Institute of Neurosciences Kolkata, Kolkata, West Bengal, Neurosciences Kolkata, West Bengal, of Institute of Medical Kolkata, West Bengal, of of Kolkata, West Bengal, India E-mail: [email protected] Background: is a in a of patients with Parkinson’s disease (PD) of is to have a genetic and human of a role of enzyme and of in of in Objectives: We to the of in and in of in PD patients with and and their association with clinical Methods: The study PD patients with PD patients and healthy was done by polymerase chain reaction Movement disorder Parkinson’s disease and were performed to the severity and Montreal cognitive assessment and were to and was than in PD patients with compared to PD patients = = The was significantly higher in PD patients with compared to of = of was than the in PD patients with compared to PD patients = = No association with clinical was observed for Conclusions: association of of and of was found in our of PD patients with from India, leading to the that could be a potential for pathogenesis. References 1. A, and of in a Parkinson’s disease 2. of and as estimated from the Abstract as a for Parkinson’s disease Department of Neurology, Institute of Medical of and of India E-mail: [email protected] Background: of α-synuclein of leading to Parkinson’s disease (PD). The to the of a on as The study the can be for Objectives: We aimed to study the of in PD using in vitro α-synuclein and Methods: Human were to study the of on in PD, using induced pluripotent stem cells (iPSCs) and for α-synuclein fibrils of and α-synuclein of on α-synuclein was in in vitro by as and and The α-synuclein PFFs treated cells were treated with and as and were to the was done to the Results: The α-synuclein cells and iPSC-derived for were into two in the were with α-synuclein treatment and in the α-synuclein treatment was by The showed higher compared to the The also showed higher expression of and in as and of with α-synuclein treatment is and the expression of and Conclusions: We have the potential of for We study to the relevant between α-synuclein and that cells with the of due to α-synuclein the and Abstract of and of signal and signal with as a factor in Parkinson’s disease Movement and Human 1Department of Neurology, Institute of Medical and Hospital, of Institute of Human and India E-mail: [email protected] Background: Parkinson’s disease (PD) in the and of that impact from indicates that may to in response and This study into the of and in response to and in PD, with a particular on the role of Objectives: We aimed to the the or the response and in for with PD by the signal with the signal Methods: with PD were using and Medical Research for their and were on with that a response and that a were to the signal by the and to and not to the the their reaction and signal and signal were and compared with the of and Results: The difference between the and the and of signal and The of signal were found to be significantly compared to the of compared with as a The of signal were also found to be significantly compared to the of compared with as a Conclusions: from the that the to a response and the of a response which is the signal by the in The study also and for the clinical of PD and our of the relationship between and Abstract study of and in patients with Parkinson’s disease and Supriyo Choudhury, Ganguly, Hrishikesh Kumar Department of Neurology, Institute of Neurosciences Kolkata, Kolkata, West Bengal, India, School, E-mail: [email protected] Background: The of for intricate is in patients with and associated to be the primary for of the and of correlation between and Objectives: We a of and among healthy controls patients with Parkinson’s disease and with Methods: of and were estimated in PD patients, and healthy by a through a of for The were to the levels and of on the by The was using a to et and the was standard clinical were for PD and patients. Results: The a in PD and patients compared to were in between the and the demonstrated a correlation with the for the assessment and of in patients and the Unified Parkinson’s Disease Rating Scale in PD patients. was an of correlation between and groups. Conclusions: The and crucial in is that the of and Abstract of as a tool to Parkinson’s disease from Department of Neurology, Institute of Medical 1Department of Institute of Medical India E-mail: [email protected] Background: is to Parkinson’s disease (PD) from in the disease. is and not Therefore, a and is to disease et al. on the in PD patients. Objectives: We aimed to on in patients with PD and if on can PD from Methods: This study was patients with PD and were PD with and with were and clinical was by Movement Disorders Society Unified Parkinson’s Disease Rating Scale of of were on with a to a of and of was The was performed through the and The was as using and controls. Results: patients with a of PD and were The of patients was in the PD and in the of was in the PD and in the of was in the PD and in the was in PD patients compared to in was in of patients in the PD compared to of patients in the and the difference was (P = Conclusions: demonstrated a in PD compared to with its and can be a noninvasive tool to between the two groups. as a in of and of in the References 1. The of Parkinson’s disease. 2. J, H, of in Parkinson’s disease by Abstract of on and in patients with Parkinson’s disease Department of Neurology, Institute of Hospital, India E-mail: [email protected] Background: the of of Parkinson’s disease of have Studies found of in of in This could in of of in the disease which can be by Objectives: This study was done to study the of by in PD patients in to healthy controls and to study the correlation between the of in PD patients and of PD, of PD, Scale for in Parkinson’s Disease Scale and Methods: study was from to We compared PD patients to healthy controls. All clinical and were by Hoehn and Movement Disorders Society Unified Parkinson’s Disease Rating Scale III), and were performed to standard using by a was performed on with by a was the of by the in the the Results: of patients were compared to controls of PD in PD of showed in PD patients compared to controls = was difference in of between PD patients and controls = showed PD compared to postural instability gait PD (P = was correlation of with the of and the of of and did not correlation with the of PD, PD Hoehn and and of in and correlation of of and of Parkinson’s disease = of in PD patients. on the compared to PIGD was correlation between and of PD, and to and References 1. R, K, et al. of the in the of Parkinson’s disease. 2. K, H, K, The in patients with Parkinson’s disease: study using Abstract in patients with Parkinson’s disease and with Department of Neurology, Institute of Medical India E-mail: [email protected] Background: of Parkinson’s disease (PD) and with by that not in the also in the can be for the of in PD and for identification in The aim of the study is to correlate the in PD and patients with their disease and cognitive Objectives: We aimed to the in patients with PD and and to correlate the in PD and patients with their disease and cognitive Methods: This study was to the role of in of patients with PD and from the patients, a of patients with PD and patients with between and and in and to the Department of Neurology, from to were and clinical were and Montreal Cognitive Assessment (MoCA) Cognitive Unified Parkinson’s disease on and and Scale were was using standard in a and in the and were Results: positive correlation was found between MoCA and and in PD patients (P and correlation was found with UPDRS and (P The did not correlation of with cognitive and Conclusions: study showed that higher and higher and associated with higher cognitive and lower UPDRS and in PD patients. References 1. S, L, et al. for 2. S, and cognitive in Parkinson’s disease. Abstract in patients with Parkinson’s disease and Department of Neurology, Institute of Medical India E-mail: [email protected] Background: may with of from of between the and the The is a the which is relevant in associated with Objectives: We aimed to study the presence and of in patients with Parkinson’s disease with and and to correlate the presence of in these patients with their disease and cognitive Methods: our a of were clinical and of these patients were We performed in these patients. were between and PD patients to the disorder and and patients. with associated peripheral were Results: study of patients was into with PD, with with and with We observed that the presence of was in PD patients. postural and showed positive correlation with Conclusions: PD patients, the presence of was associated with increased of postural and Abstract in of Parkinson’s disease Vikram Department of Neurology, Institute of and Neurosciences, 1Department of and Institute of and Neurosciences, India E-mail: [email protected] Background: The Parkinson’s disease and postural instability and gait (PIGD) may have in which to the is as a noninvasive to study by the and Objectives: we aimed to the in the profile between patients with and Methods: was for patients with and patients with PIGD PD, were in the and Parkinson’s Disease by the for and the Institute of and Neurosciences, India, from to and All clinical assessment the disorder Parkinson’s disease and disorder and with with enhanced to the was to be Results: was difference in of and between and PIGD patients. the PIGD a significantly lower higher and Hoehn and patients with PIGD significantly higher for and in the of in compared to PIGD of the and Conclusions: PIGD is to be the with a higher of to we observed and in This to be in to understand the Abstract and of in Parkinson’s disease and Department of Neurology, Institute of Medical India E-mail: [email protected] Background: This study was aimed to in patients with Parkinson’s disease (PD) and and to if can be as a for of Objectives: We aimed to and in PD and patients. Methods: of were We in these patients. were between and PD patients to the disorder and with and patients. We patients with and Results: We observed that was in the of PD patients compared to patients Conclusions: the of our study showed in the of PD patients compared to patients. of these and the noninvasive of we its for PD as well as disease of a be as an to in assessment of gait and in patients with Parkinson’s Disease and Movement India E-mail: [email protected] Background: and and gait of and of is a with a of than in healthy is an clinical which to Objectives: We aimed to to a of the relationship between assessments with and in with Methods: We performed and for with and the were documented for Results: patients disease with of disease were was correlation between and among PD, and PD patients showed correlation between and to PD patients, patients (P and increased to PD patients, PD patients increased and not Conclusions: our study correlation between and in patients with we a between and among patients with determined by profile of patients with from India Vikram Department of Neurology, Institute of and Neurosciences, of of India E-mail: [email protected] Background: in the a of Parkinson’s disease. The clinical phenotype may from Parkinson’s disease (PD) to or gait as the Objectives: The aim of our study is to the clinical and genetic profile of patients of and to correlation with genetic Methods: patients with PD were and from our of patients with PD clinical and genetic of the patients were through a as and Results: patients = with a of and disease of were recruited. All and disorder were in a All were on with found in and in two patients. was and patients was in all, two patients and tremor with phenotype in patients and in patients The Unified Parkinson’s Disease Rating Scale in the and on were and with an of All of the performed in was sequencing in were Conclusions: as an PD with have with and We in the associated with the disease in the in the 1. R, H, Li Y, Y, H, et al. of the in patients with disease. 2. R, L, and the of Parkinson’s disease. Psychiatry Abstract of patients with Parkinson’s disease and from a in India Vikram Department of Neurology, Institute of and Neurosciences, of of India E-mail: [email protected] Background: Parkinson’s disease (PD) to is of the of genetic dominant PD with PD phenotype and to be tremor the and on the Objectives: The aim of our study is to the clinical and genetic profile of patients of PD and to correlation with genetic Methods: patients with PD were and from our of patients with PD clinical and genetic of the patients were through a Results: patients with a of and a disease of were was in and lower in the patients was the All were on with found in and in patients. patients of patients and cognitive patients The in Unified Parkinson’s Disease Rating Scale on was sequencing two two and the of was in the of of the in in the PD with PD in the and was the of the patients and were with the of the were of Abstract and longitudinal study of cognitive in Parkinson’s disease Department of Neurology, Institute of Human and India E-mail: [email protected] Background: Cognitive in Parkinson’s disease (PD) is the which to and in and the which in the as Objectives: We aimed to study the of cognitive and its in patients with Methods: The of study was PD patients were from disorder were for cognitive using Montreal Cognitive Assessment and were for to an of for in cognitive were in by Results: of patients were were into PD and PD the PD were patients cognitive patients with patients cognitive patients cognitive a of and to PD of patients the PD of the patients cognitive the and cognitive in of and correlation with cognitive and were the cognitive by and the Conclusions: Cognitive is in and genetic to patients with a of cognitive and of PD with cognitive of to the disease and the of patients. Abstract The profile of of Parkinson’s disease Vikram Department of Neurology, Institute of and Neurosciences, 1Department of and Institute of and Neurosciences, of Institute of and Neurosciences, India E-mail: [email protected] Background: with Parkinson’s disease (PD) can with cognitive from to have been between the of PD and postural instability and gait with the with Objectives: This study to the profile of and PIGD in our of patients. Methods: was for patients with and patients with PIGD PD were in the and Parkinson’s Disease by the for and the Institute of and Neurosciences, India, from to and a All clinical assessment the Movement Disorders Society Unified Parkinson’s Disease Rating Scale and the Rating Scale The was using the for in Parkinson’s assessment of and cognitive for the was with of as a with to be Results: was difference in the assessment or between and PIGD patients. the PIGD a significantly lower of higher and and Hoehn and was for of the PIGD significantly higher The PIGD also higher for the of and The a in in patients the in was also in patients with have in compared to PIGD patients. This is as with of in PIGD patients. the two and on patients in to healthy controls and an of patients. be to these Abstract profile of the of Parkinson’s disease Vikram Kumar Department of Neurology, Institute of and Neurosciences, 1Department of and Institute of and Neurosciences, India E-mail: [email protected] Background: Parkinson’s disease (PD) can be on into PD and postural instability and gait have been to between the the of of in to the severity of Objectives: This study to the of and PIGD in our of patients and the in between subtypes. Methods: was for patients with PD were in the and Parkinson’s Disease by the for and the Institute of and Neurosciences, India, from to All clinical assessment the Movement Disorders Society Unified Parkinson’s Disease Rating Scale and the Rating Scale and were into or PIGD on the of for was with of as a with to be Results: the = of patients were of = were of PIGD and were of the was difference in the or between and PIGD patients. PIGD patients a significantly of higher Hoehn and and was for of the PIGD significantly higher and higher for the of and with PIGD have a significantly higher of compared to patients with the in the of and is to in patients with Abstract of in Parkinson’s disease in West Hospital, West Bengal, India E-mail: [email protected] Background: in Parkinson’s disease patients with a of and which the of Objectives: We aimed to study the clinical profile of in patients. Methods: All patients the Parkinson’s Disease Society for Parkinson’s disease the a in India, the from to were All of and were All patients were and was were documented using the of Parkinson’s and Movement Hoehn and was for Results: of patients were with the study were and were to the of and the of was from 1 to with an in and 2 Hoehn and in were with and the of the patients or Conclusions: were in patients study with and the References 1. et al. and of Parkinson’s disease: on the of the 2. A, Parkinson’s disease. Abstract of and in patients with and Parkinson’s disease Vikram Kumar Department of Neurology, Institute of and Neurosciences, India E-mail: [email protected] Background: Parkinson’s disease (PD) is a neurodegenerative disease that is due to of in the is by and of and the of of patients and the burden. Objectives: We aimed to the and in patients with PD and PD Methods: We patients of and and clinical were was from the They were for by the using and of or 1 was for an or a was using the was to and The from to and a was as The were and Results: and patients and patients and patients patients and patients did not an of patients in on of The were and in the and The of in and patients were and in PD patients were and and in PD patients were and The in the and were and The in the and

Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.