Fecal Metabolites_original.XLSX

Fecal and serum samples were maintained at -80^◦C until metabolic profiling was performed. Global non-targeted mass spectrometry metabolomics analysis was performed at Metabolon, Inc (Metabolon, Inc, Durham, NC), a commercial supplier of metabolic analysis, which has developed a platform that integrates chemical analysis (including identification and relative quantification), data-reduction, and quality assurance. To maximize compound detection and accuracy, 3 separate analytical methods were utilized including ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-LC-MS) in both positive and negative ion modes and gas chromatography/mass spectrometry (GC-MS) (40, 41). Following receipt, samples were inventoried, accessioned into the Metabolon Laboratory Information Management System (LIMS), assigned a unique identifier, and stored at -80^◦C until analyzed. Sample preparation was performed by the automated Mircolab STAR system (Hamilton Company, Salt Lake City, UT, USA). Extraction solvents (<i>i.e.</i>, methanol containing recovery standards) were added to each sample. <i> </i>Extraction was carried out by shaking using a Geno/Grinder 2000 (Glen Mills, Clifton, NJ) followed by centrifugation. The resulting extract was divided into 5 fractions: 2 for analysis by 2 separate reverse phase (RP) LC/MS methods with positive ion mode electrospray ionization (ESI), 1 for analysis by RP LC/MS with negative ion mode ESI, 1 for GC/MC analysis, and 1 reserve aliquot. Samples were placed on a TurboVap® (Zymark, Midland, Ontario, Canada) to remove organic solvent and were stored overnight under nitrogen before preparation for analysis. <b> </b> All UPLC-MS/MS methods utilized a Waters Acquity UPLC (Walters, Milford, MA, USA) and a Q-Exactive high resolution/accurate mass spectrometer interfaced with heated electrospray ionization (HESI-II) source and Orbitrap mass analyzer operated at 35,000 mass resolution (Thermo Scientific, Walthram, MA, USA). Sample extracts were reconstituted in solvents compatible to each method employed. The MS analysis alternated between MS and data-dependent MSⁿ scans using dynamic exclusion. The scan range varied slighted between methods but covered mass to charge ratios of 70 to1000 <i>m/z</i>. Raw data were archived and extracted. For GC/MS<i>, </i>derivatized samples were separated on a 5% phenyldimethyl silicone column with helium carrier gas. Data were analyzed on a Thermo-Finnigan Trace DSQ MS (Thermo Fisher) operated at a unit mass resolving power with electron impact ionization. Detection, identification, integration, and clustering of all ion features into individual compounds was performed using a library of conserved chemical standards using a software developed by Metabolon (42). Authenticated standards within the library contain retention time/index (RI), mass to charge ratio (<i>m/z)</i>, and chromatographic data (including MS/MS spectral data). At present, 3,000 commercially available purified standards are registered into the LIMS for distribution to both the LC and GC platforms for determination of their analytical characteristics. Compound abundance was quantified by calculation of area under the curve for the quantification ion of the compound. Quality control measurements were used to individually check all compounds.

Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.