MP59-07 STRICT GLUCOSE CONTROL AND ELIMINATION OF NLRP3-INDUCED INFLAMMATION PREVENTS DIABETIC BLADDER DYSFUNCTION IN THE FEMALE AKITA MOUSE MODEL
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Résumé
You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research & Pathophysiology (MP59)1 May 2024MP59-07 STRICT GLUCOSE CONTROL AND ELIMINATION OF NLRP3-INDUCED INFLAMMATION PREVENTS DIABETIC BLADDER DYSFUNCTION IN THE FEMALE AKITA MOUSE MODEL Shelby N. Harper, Michael Odom, Francis M. Hughes, Huixia Jin, and Todd Purves Shelby N. HarperShelby N. Harper , Michael OdomMichael Odom , Francis M. HughesFrancis M. Hughes , Huixia JinHuixia Jin , and Todd PurvesTodd Purves View All Author Informationhttps://doi.org/10.1097/01.JU.0001009476.18935.3b.07AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Diabetic bladder dysfunction (DBD) is a prevalent complication with no specific treatment option. Logically, strict regulation of blood glucose should reverse dysfunction, but the Epidemiology of Diabetes Interventions and Complications study found strict control ineffective. However, it is possible that strict glucose control, initiated early, may prevent DBD. In this study, we examine the effect of early glucose control (strict and poor) on the development of DBD in the female Akita mouse, a Type 1 diabetic model. Female Akitas develop signs of overactive bladder (OAB) due to inflammation mediated by the NLRP3 inflammasome. Given the NLRP3-dependency of this model, we have expanded this study to evaluate eliminating NLRP3 as adjunct therapy to glucose control. METHODS: Akita mice were bred NLRP3+/+ or NLRP3-/-. Only females were used. At 6 weeks of age, diabetics received either no glucose control or insulin pellets (s.c., Linshin, Toronto) designed to poorly (0.05 U/day) or strictly (0.125 U/day) control blood glucose. At week 15, blood glucose (glucometer), HbA1c levels (ELISA), bladder inflammation (Evans blue) and bladder function (urodynamics) was assessed. RESULTS: Blood glucose (A) of uncontrolled diabetics was 244±20 mg/dl, which was reduced in the poorly controlled diabetics (186±13 mg/dl) and strongly reduced in the strictly controlled group (131±16 mg/dl). Nondiabetics averaged 113±5 mg/dl. These levels were not affected by deletion of NLRP3 and were reflected by HbA1c levels. Bladder inflammation (B) directly correlated with glucose control and was eliminated in the NLRP3-/- groups. Urodynamics found markers of overactivity (reduced void volume, etc.) in uncontrolled diabetics which improved in the poorly controlled group. Strict control eliminated dysfunction, showing that effective regulation of blood glucose, initiated early, can prevent DBD. In the NLRP3-/- mice, no bladder dysfunction developed. This suggests that elimination of NLRP3-induced inflammation will prevent DBD, an approach that may be an important adjunct to glucose control. CONCLUSIONS: Both early-initiated strict glycemic control and NLRP3 elimination can effectively prevent DBD, suggesting hyperglycemia acts through NLRP3-induced inflammation to trigger DBD. Download PPT Source of Funding: NIH-RO1 DK117890, NIH-K12 DK100024 © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e959 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Shelby N. Harper More articles by this author Michael Odom More articles by this author Francis M. Hughes More articles by this author Huixia Jin More articles by this author Todd Purves More articles by this author Expand All Advertisement PDF downloadLoading ...
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,001 | 0,001 |
| Méta-épidémiologie (sens large) | 0,001 | 0,002 |
| Bibliométrie | 0,002 | 0,001 |
| Études des sciences et des technologies | 0,001 | 0,001 |
| Communication savante | 0,001 | 0,001 |
| Science ouverte | 0,001 | 0,001 |
| Intégrité de la recherche | 0,002 | 0,004 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,041 | 0,007 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».