PD56-08 EFFECT OF SODIUM THIOSULFATE PRE-TREATMENT ON RENAL ISCHEMIA-REPERFUSION INJURY
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You have accessJournal of UrologyTransplantation & Vascular Surgery II (PD56)1 May 2024PD56-08 EFFECT OF SODIUM THIOSULFATE PRE-TREATMENT ON RENAL ISCHEMIA-REPERFUSION INJURY Pierce Nelson, George Dugbartey, and Alp Sener Pierce NelsonPierce Nelson , George DugbarteyGeorge Dugbartey , and Alp SenerAlp Sener View All Author Informationhttps://doi.org/10.1097/01.JU.0001008928.01012.0d.08AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Ischemia-reperfusion injury (IRI) is a major cause of impaired graft function following kidney transplantation. We recently showed that adding sodium thiosulfate (STS), a hydrogen sulfide (H2S) donor, to University of Wisconsin (UW) solution protects against renal IRI. However, it is unknown if treatment of kidney donors with H2S before ischemia can also protect against renal IRI. We hypothesize that STS treatment of kidney donors before graft procurement will protect renal grafts against IRI and thereby improve graft function. METHODS: Rat (NRK-52E) and human (HK-2) kidney epithelial cells were exposed to 24 (NRK-52E) or 18 (HK-2) hours of 37°C hypoxia and 24 hours of reoxygenation. NRK-52E cells were treated with 150 μM STS for 2 hours before hypoxia while HK-2 cells were treated with 150 μM, 500 μM, 1 mM, 5 mM, or 10 mM of STS for 24 hours before hypoxia. Cell viability was assessed via flow cytometry. In our in vivo model, donor rats were injected with saline or 2.4 mg STS/kg 30 minutes before organ procurement. Renal grafts were stored in UW or UW+150 μM STS at 4°C for 24 hours and transplanted into recipient rats, which were monitored until post-operative day (POD) 3 and sacrificed to measure blood, urinary, and tissue markers of graft function and injury. RESULTS: STS pre-treatment significantly improved cell viability (p<0.01) in NRK-52E cells while preliminary data suggest that STS pre-treatment improves cell viability in HK-2 cells. Our preliminary in vivo data suggest that STS pre-treatment and supplementation to UW solution improve recipient kidney function. CONCLUSIONS: Our results show that STS pre-treatment is protective in in vitro models of warm IRI. Preliminary data from our in vivo model suggest STS pre-treatment may protect against transplantation-induced renal IRI, warranting further study. Download PPTDownload PPTDownload PPT Source of Funding: Canadian Institutes of Health Research Canada Graduate Scholarship - Master's © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e1205 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Pierce Nelson More articles by this author George Dugbartey More articles by this author Alp Sener More articles by this author Expand All Advertisement PDF downloadLoading ...
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|---|---|---|
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| Bibliométrie | 0,000 | 0,000 |
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