DOX-Couped Polymeric Micelles as a State-of-the-Art Strategy Against Triple Negative Breast Cancer
Notice bibliographique
Résumé
Triple-negative breast cancer (TNBC), a unique type of breast cancer, is defined by the absence of ER, PR, and HER-2 receptors in the tumor.This particularly aggressive form of breast cancer, TNBC, shows early signs of resistance to chemotherapy [1].One significant challenge with this subtype is its less favorable outlook compared to other types of breast cancer, resulting in lower overall survival rates, frequent relapses, and increased mortality.Current treatments for Triple-Negative Breast Cancer (TNBC) typically include chemotherapy, surgery, and radiation therapy.However, the use of these treatments remains limited due to high systemic toxicity, the development of chemotherapy resistance, tumor heterogeneity, and a high risk of metastasis [2].Therefore, new, and effective treatment approaches should be developed for TNBC.Doxorubicin (DOX) remains the primary chemotherapy agent employed in the traditional treatment of triple-negative breast cancer (TNBC).Nevertheless, its clinical use is suboptimal due to issues such as drug resistance, non-discriminatory distribution, cardiac toxicity, limited solubility and restricted penetration [3].Hence, the development of a drug delivery system that decreases the harmful effects of drug and enhances penetration is essential for the successful treatment of TNBC.With the aim of introducing treatment for TNBC, we have engineered polymeric nanoparticles that are coupled with doxorubicin.In our research, we effectively produced a new negatively charged SPMA/PMMA polymer using RAFT polymerization.Nanoparticles were generated via the nanoprecipitation technique.To create a complex between nanoparticles and DOX, we utilized electrostatic binding.Based on Dynamic Light Scattering (DLS) and ζ-potential assessment, our nanoparticles size increased slightly from 134.3 ± 1 nm to 189.7 ± 10 nm, whereas the charge of the particles were increased from -46.9 mV to -15 mV.Complexes were formed at different weight ratios (1:1, 1:2, 1:5), and the complex with the highest binding efficiency and the smallest size is 1:1 complexation ratio.Upon the formation of a complex between doxorubicin and the nanoparticles (DOX NPs), there was a slight increase in the size of the nanoparticles, growing from 134.3 ± 1 nm to 189.7 ± 10 nm.Simultaneously, the charge of the nanoparticles shifted from -46.9 ±3 mV to -15 ±4 mV.The critical micelle concentration (CMC) was established at 17 μg/mL, and the binding efficiency was assessed at 64.5 ±2 % based on the measurement of free doxorubicin absorbance in the supernatants.While bare NPs did not exhibit significant cytotoxicity, the presence of DOX within the nanocarriers reduced the viability of breast cancer cells, and this effect was observed similarly to free DOX treatment.In line with these results, our biocompatible carrier system, which we designed, successfully delivered DOX to breast cancer cells, and demonstrated its potential as an anti-cancer agent by reducing cell viability.
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Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,001 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,001 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».