Piloting environmental DNA for fisheries monitoring in the upper Peace River watershed
Notice bibliographique
Résumé
Applications of environmental DNA (eDNA) represent one of the most significant recent advances in aquatic species monitoring. eDNA has the potential to dramatically increase the effectiveness and efficiency of fisheries monitoring across large geographic areas and particularly for species that have traditionally been difficult to survey. The Williston Reservoir and its surrounding watershed in northern British Columbia is a vast and heavily disturbed aquatic ecosystem where fish stocks have been difficult to monitor with traditional survey techniques. Although eDNA has many potential benefits, the validation process required in a novel environment can be a barrier to adoption. Primer choice and validation, study design and laboratory workflows are critical considerations for eDNA studies and must be evaluated when developing a novel approach in a particular geographic area. My research advanced the validation of species-specific eDNA assays for six prominent pelagic species in the Williston Reservoir and a generic, metabarcoding primer set that was effective in detecting the diversity of fish in the surrounding streams and rivers. The species-specific approach included the validation of four published assays and the development of novel assays for detecting lake trout (Salvelinus namaycush) and peamouth (Mylocheilus caurinus). Lake trout and bull trout (Salvelinus confluentus) are very closely related species that are sympatric throughout much of northwestern North America. The development of a novel lake trout assay that did not cross amplify bull trout eDNA is an important achievement for species-specific eDNA monitoring. Reservoir samples were collected from above and below the thermocline during the summer when the reservoir was stratified to identify species-specific patterns of eDNA distribution throughout the water column. Species detections in the reservoir were comparable for species-specific and metabarcoding assays when compared against gillnet catches in the reservoir. Abundant species that exhibit diel vertical migrations were detected by eDNA and gillnets at all depths. eDNA copy numbers detected were higher for samples with greater abundance and biomass of each species caught in the gillnets. Samples collected from tributaries around the reservoir were tested with metabarcoding primers and species detections were compared to results from a species-specific assay for Arctic grayling and a snorkel survey. Metabarcoding results were comparable to those from the Arctic grayling eDNA study and the snorkels survey, although the metabarcoding methods in this study were less sensitive due to a reduced amount of sample replication. Piloting eDNA in the Williston Reservoir and surrounding watershed has provided valuable insights into eDNA sampling design and the overall workflow for future eDNA studies in the region.,
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,004 | 0,006 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,002 | 0,002 |
| Études des sciences et des technologies | 0,001 | 0,001 |
| Communication savante | 0,002 | 0,002 |
| Science ouverte | 0,002 | 0,002 |
| Intégrité de la recherche | 0,001 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,002 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».