Abstract B033: Characterizing the spatial transcriptomic landscape of Osteosarcoma from diagnosis to relapse
Notice bibliographique
Résumé
Abstract Introduction This study aims at elucidating mechanisms of immune infiltration in osteosarcoma (OSA) to identify novel therapeutic strategies. Methods Here, joint analysis of spatial transcriptomics (ST, Visium) data from 26 formalin-fixed paraffin embedded OSA samples at diagnosis (n=9) and relapse (n=17, MAPPYACTS NCT02613962 & OS2006 NCT00470223) from 19 donors (including 5 diagnosis/relapse pairs) was performed using the CellsFromSpace unbiased reference-free signal deconvolution and analysis workflow. This methodology enabled the definition of the topology and the molecular signatures of the cancerous, stromal and immune components across all samples, and allowed for the phenotyping of individual tumors based on cancer subset composition. Spatial colocalization analysis was performed using Lee’s bivariate spatial association measure of different cell populations, enabling the characterization of tumor-infiltrating immune cells found across samples. Similar ST analysis of Patient-derived xenograft (PDX) samples was performed on fresh-frozen samples. Subsequent separation of human and mouse reads using the Xenome algorithm allowed for a clear dissection of the cancer vs. microenvironment compartments. Preliminary signature validation on larger cohorts was performed on bulk RNAseq samples from the OS2006 and MAPPYACTS cohorts. Results Our joint spatial transcriptomics analysis of 26 primary and relapse tumors revealed 64 transcriptomic signatures associated with cancer cells, some of which were shared across multiple samples. These signatures could be consolidated into 18 broad phenotypic categories frequently detectable in various samples, showcasing significant intratumoral heterogeneity within the cancerous compartment. By averaging the composition per sample, we found that samples clustered based on the relative intratumoral abundance of cancer phenotypes, indicating the presence of at least two main differentiation archetypes in OSA: “High intratumoral heterogeneity” and “Undifferentiated.” Additionally, spatial niche analysis of immune cells within tumors identified immune populations consistently associated with tumors across samples. This analysis revealed a specific myeloid lineage capable of infiltrating tumors in all samples, likely mediated by chemotactic signals linked to cancer differentiation, as suggested by ligand-receptor analysis. To validate these findings, PDX models of OSA were analyzed, confirming the presence of a similar phenotype of tumor-infiltrating myeloid cells and underscoring the relevance of PDX models in OSA research. Furthermore, preliminary analyses querying the transcriptomic signature of infiltrating myeloid cells in larger bulk RNAseq cohorts revealed promising associations with patient outcomes, detectable even at diagnosis. Conclusions Our research elucidates patterns of intratumoral phenotypic heterogeneity in OSA and identifies the primary immune cell populations specifically infiltrating OSA tumors. This paves the way for developing novel therapies by leveraging these cellular tropism mechanisms. Citation Format: Gaël Moquin-Beaudry, Maria Eugenia Marques Da Costa, Hanane Zair, Corentin Thuilliez, Pierre Khneisser, Nicolas Signolle, Jean-Yves Scoazec, Birgit Geoerger, Nathalie Gaspar, Antonin Marchais. Characterizing the spatial transcriptomic landscape of Osteosarcoma from diagnosis to relapse [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Advances in Pediatric Cancer Research; 2024 Sep 5-8; Toronto, Ontario, Canada. Philadelphia (PA): AACR; Cancer Res 2024;84(17 Suppl):Abstract nr B033.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,001 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,001 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,001 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,002 | 0,001 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».