Abstract A034 LIBERTY: LIquid Biopsy for rElapsed/RefracTorY neuroblastoma - a pilot study of characterization of circulating tumor DNA alterations in patients with relapsed and refractory neuroblastoma using a clinically validated liquid biopsy platform
Notice bibliographique
Résumé
Abstract Introduction Detection of tumor-derived targetable alterations from peripheral blood samples represents a unique opportunity for patients (pts) with hard-to-biopsy neuroblastoma (NBL). The overall aim of LIBERTY is to describe the molecular alteration landscape identified by circulating tumor DNA (ctDNA) profiling in pts with relapsed/refractory NBL by providing rapid turnaround results to Canadian patients, using a clinically validated commercial liquid biopsy platform. Methods LIBERTY is a prospective non-interventional study of molecular profiling of ctDNA for pts with relapsed, refractory or not amenable to biopsy NBL, treated in a Canadian oncology center. The Tempus next-generation sequencing xF+ assay is performed on ctDNA extracted from blood collected at a single timepoint. The 523-gene panel includes single nucleotide variants, insertions and deletions, copy number variants and chromosomal rearrangements, using 30-50 ng of input DNA. An analytically validated report of genomic findings is returned to the study team and may be used to inform treatment recommendations. Results To date, 15 pts have been enrolled (6 male), with a median age of 6.7 y (range, 2.8-24.6). Disease status at enrolment was primary refractory/progressive NBL (n=2), first relapse (n=4), second or greater relapse (n=9). Nine pts were primarily treated at the Hospital for Sick Children. Remote consent was performed for remaining 6 pts, with blood sample being collected at and shipped from their institution to TEMPUS. Thirteen of 15 (86.6%) pts had been previously enrolled in another pediatric precision oncology program (KiCS (n=10), PROFYLE (n=1), KiCS/PROFYLE (n=2)), which provided sequencing results of tumor tissue-derived DNA. Median turnaround time was 13 days from blood draw to available report (range, 9-27). ctDNA extraction and sequencing led to generation of a research report for 14/15 pts. Biologically relevant variants, as defined by the TEMPUS knowledge database as variants with functional significance or association with disease state, and may have relevant therapeutic, prognostic or diagnostic evidence, were reported for 8/14 (57%), with a median variant allele fraction of 10.6% (range, 0.2%-47.4%). At least one biologically relevant variant was a novel finding for 6 pts (not in previous tumor tissue sequencing (n=5); no previous sequencing (n=1)) and in 3 pts, was deemed therapeutically actionable – variants in PIK3R1, RET, CHEK2 for SK-0010; ALK for SK-0013; PTPN11 for SK-0015. For one pt with ALK p.F1174L, ctDNA sequencing led to a change in therapy in the context of first relapse. Conclusion Using a commercially available liquid biopsy tool within the confines of a research study for pts with relapsed/refractory NBL is feasible in an expansive country like Canada. Remote consent and shipping of blood samples directly from pt’s institution facilitated access from across the country. Enrolment in LIBERTY gave pts timely access to ctDNA next-generation sequencing and led to identification of novel findings with implications for therapy. Citation Format: Sarah Cohen-Gogo, Gabriel Revon-Riviere, Jathishinie Jegathisawaran, Adam Shlien, Anita Villani, Meredith Irwin, Daniel A. Morgenstern. LIBERTY: LIquid Biopsy for rElapsed/RefracTorY neuroblastoma - a pilot study of characterization of circulating tumor DNA alterations in patients with relapsed and refractory neuroblastoma using a clinically validated liquid biopsy platform [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Advances in Pediatric Cancer Research; 2024 Sep 5-8; Toronto, Ontario, Canada. Philadelphia (PA): AACR; Cancer Res 2024;84(17 Suppl):Abstract nr A034.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,001 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,001 | 0,001 |
| Communication savante | 0,001 | 0,000 |
| Science ouverte | 0,001 | 0,001 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,003 | 0,001 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».