Caveolin 1 ameliorates nonesterified fatty acid–induced oxidative stress via the autophagy regulator beclin 1 in bovine mammary gland epithelial cells
Notice bibliographique
Résumé
High blood concentrations of nonesterified fatty acids (NEFA) during ketosis enhance uptake by the mammary gland and impair autophagy while causing oxidative stress. Caveolin 1 (CAV1) is closely related to autophagy and plays a role in regulating oxidative stress. The aim of this study was to explore the potential role of CAV1 on oxidative stress and autophagy during a high-NEFA challenge in the immortalized bovine mammary epithelial cell line (MAC-T). Mammary gland tissue biopsies and blood samples were collected from healthy (n = 15) and clinically ketotic (n = 15) Holstein cows at 3 to 10 (average = 6) days in milk. Compared with healthy cows, ketotic cows had lower DMI, daily milk yield, and serum glucose, and greater serum NEFA and BHB, accompanied by greater milk fat and lower milk protein. Malondialdehyde (MDA) was greater, but activities of superoxide dismutase (SOD), catalase, and glutathione peroxidase were lower in cows with clinical ketosis. A lower protein abundance of CAV1, beclin 1, autophagy relative gene 5 (ATG5), and microtubule-associated protein 1 light chain 3 (LC3) as well as greater protein abundance of sequestosome-1 (SQSTM1, also called p62) were detected in mammary tissue of cows with clinical ketosis. In vitro, the MAC-T cells were treated with 0, 0.6, and 1.2 mM NEFA for 12 h or treated with 1.2 mM NEFA for various lengths of time (0, 0.5, 1, 2, 4, 8, 12, and 24 h). Compared with 0 mM NEFA, protein abundances of CAV1, beclin 1, ATG5, and LC3 were greater in the MAC-T challenged with 0.6 mM NEFA but lower in the 1.2 mM NEFA group. Protein abundance of p62 was lower with 0.6 mM NEFA but higher with 1.2 mM NEFA. In response to increasing doses of NEFA, mRNA abundance of CAV1, total antioxidant capacity and SOD activity decreased, whereas the levels of reactive oxygen species and MDA content increased. The protein abundances of CAV1 and beclin 1 peaked at 0.5 h, the protein abundances of ATG5 and LC3 peaked at 1 h, and these proteins start to fade away at later time points under NEFA treatment, resulting in both linear and quadratic effects. The protein abundance of p62 decreased, reaching a nadir at 4 h in both a linear and quadratic manner. The silencing of CAV1 in MAC-T cells aggravated the 1.2 mM NEFA-induced decrease in beclin 1 expression, impaired autophagy, and increased severe oxidative stress, whereas the overexpression of CAV1 alleviated these effects. Pretreatment of MAC-T cells with beclin 1 small interfering RNA (si-beclin 1) and the overexpression of CAV1, followed by challenge with 1.2 mM NEFA, resulted in reversed CAV1-induced autophagy, thereby aggravating oxidative stress. Overall, these data suggest that CAV1 protects bovine mammary epithelial cells from NEFA-induced oxidative stress through enhancing the expression of beclin 1 and activating autophagy.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,001 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».