Contribution of biofilm and Cell Surface Hydrophobicity in the resistance of Pseudomonas aeruginosa from some Healthcare settings of Yaounde
Notice bibliographique
Résumé
Antibiotic resistance (ABR) is one of the priority problems around the world which affects the evolution of infectious diseases and leads to economic losses with a considerable impact on increased mortality and morbidity in low-resource countries. One of the bacteria most involved in resistance is Pseudomonas aeruginosa. It is a bacteria belonging to the ESKAPE group, for which there is an urgent and critical need for antibiotic development. The circulation of this bacteria in several countries has been the cause of many infections affecting all ages, patients and healthcare staff, and has promoted the spread of resistance in environmental and animal health. To investigate the determinants of resistance in Pseudomonsa aeruginosa, the aim of this work was to assess the correlation between biofilm, Cell Surface Hydrophobicity (CSH) and antibiotic resistance in clinical isolates of Pseudomonas aeruginosa circulating in Yaounde. Methods: We conducted a cross sectional study from March 2019 to October 2021. We collected various samples including pus, urine, lochia, wound, bedsore and blood, from patients of the General Hospital of Yaounde (GHY), Central Hospital of Yaounde (CHY), University Teaching Hospital of Yaounde (UTHY) and Centre Pasteur du Cameroun (CPC). The hydrophobicity of the bacterial surfaces was determined by measuring the percentage of adhesion to xylene. Biofilm formation was assessed using the TCP method. The correlation between CSH, biofilm production and ABR were determined. Results: We included 300 patients in this study. The most represented patients were men (50.67%). P. aeruginosa was isolated from the four settings and the prevalence was 10%. A large proportion of P.aeruginosa were biofilm producer with 30% being strong biofilm producer, 53.33% were weak biofilm producer and 16.67% were non-biofilm producers. Twenty percent of P. aeruginosa were highly hydrophobes while, 23.33% were moderately hydrophobes and 56.67 were low hydrophobes. The activities of most antibiotics were negatively correlated with biofilms and hydrophobicity. Isolates from lochia and wounds were biofilm producers and showed multidrug resistance while isolates from urine and pus was very hydrophobic. Conclusion: These findings should serve as evidence base that biofilms and hydrophobicity are factors that contribute to antibiotic resistance in Pseudomonas aeruginosa. This demonstrates the need to consider these virulence factors, as well as the nature of the sample or excipient, during patient treatment, especially during empirical antibiotic therapy.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,017 | 0,012 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,003 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».