Detection of CTDNA after neoadjuvant chemotherapy predicts distant relapse-free survival, local and distant recurrence in TNBC: Findings from Tricia study
Notice bibliographique
Résumé
Introduction: Exosomes are membranous vesicles (40-150 nm) that carry biological information to distant tissues, being able to regulate several tumor processes.They are a valuable tool that may represent a turning point in the clinical management of non-small cell lung cancer (NSCLC).Goals: Analyze plasma NSCLC derived exosomes cargo for searching new biomarkers that could improve NSCLC clinical management.Materials and methods: Plasma samples were collected from 36 patients with advanced-stage NSCLC.Exosomes were isolated from plasma by ExoGAG.Exosomal RNAs were extracted using exoRNeasy Midi Kit and were RNA integrity and concentration were assessed using the Agilent RNA Pico Chip.A transcriptomic multiplexed analysis using nCounter Low RNA Input Amplification Kit was performed to analyze mRNA of plasma-derived exosomes.Particularly, a human custom panel consisting of 30 genes was used to analyze plasma-derived EVs, including biomarkers associated with CSCs population, immune and drug response, proliferation and cell cycle, and other genes associated with lung cancer.Non-parametric Mann-Whitney U and Kruskal-Wallis tests were used to compare continuous variables.Prognostic value was determined by Kaplan-Meier curves.A p-value <0.05 was considered statistically significant.Results: Exosomal RNA fragments were in the range of 30-200 nt.Out of the 30 probes included in the custom panel, only 23 showed hybridization signals in more than one patient (3 probes corresponding to housekeeping genes were included).Out of the 36 plasmatic exosome samples, only 32 successfully completed the entire amplification, hybridization, and normalization process.Transcriptomic analysis of plasma NSCLC exosomes revealed that CD24 was significantly associated with gender and histology.Moreover, markers like MICA, RIOK3, S100A2 and MMP9 were significantly associated with histology, stage, mutational status, or response to therapeutic approaches, respectively.No significant association between these genes and the patients' RFS/OS (p>0.05) was found.Conclusions: In conclusion, our study aimed to analyze plasma-derived exosomes from NSCLC patients for identifying potential biomarkers.Exosomal RNA analysis revealed associations between certain genes and different clinical factors, though no significant correlations were found with patient survival.Further research is needed to validate these findings.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».