(237) INVESTIGATING THE EFFECTS OF ACTINIDIN AS A COLLAGENASE ON HUMAN PEYRONIE’S DISEASE CELLS
Notice bibliographique
Résumé
Abstract Introduction Peyronie's Disease (PD) is a condition characterized by a connective tissue disorder that affects the penile tunica albuginea, leading to the formation of collagen-rich plaques. These plaques can cause penile curvature, painful erections, and erectile dysfunction. With the withdrawal of Collagenase C. histolyticum injections from the Canadian market, treatment options for PD have become limited. Actinidin, a soluble enzyme capable of breaking down various forms of collagen and fibrinogen, has shown effectiveness in reducing collagen in human in vitro cell models. Objective To assess the cytotoxicity of actinidin and evaluate its efficacy in reducing intercellular bound collagen in a 2D cellular human Peyronie's disease plaque models. Methods A 2D cellular model was cultured using human fibroblast cells isolated from PD tissues (N ≥ 4). Actinidin treatment was prepared using isolated actinidin powder in media, filtered, and adjusted to pH 7. Cells were treated with 10 ng/ml of TGF-β to induce a PD-like cellular model for 24 hours and then exposed to actinidin concentrations ranging from 1 mg/ml to 20 mg/ml. Media-only and TGF-β-only controls were included. Cell proliferation was assessed at 24 hours at various actinidin concentrations using an MTT assay. Collagen I levels were quantified through western blot analysis after 24 hours of treatment. Results were analyzed using oneway Anova analysis. Results Results from both the cell proliferation and collagen I quantification revealed that TGF-b significantly increases cell proliferation (P = 0.042) and collagen I levels (P = 0.018). After treating the cells with various concentrations of actinidin, the cell proliferation assay revealed that cell viability at 20 mg/ml was significantly lower (P = 0.038) compared to the TGF-β control. Additionally, a trend was observed where cell proliferation appeared to slightly decrease at higher concentrations of actinidin, although there was no significant difference in cell viability between the other treated concentrations and the TGF-β control. Western blot analysis showed that Collagen I levels were significantly reduced after 24 hours of actinidin treatment at concentrations of 10 mg/ml and 20 mg/ml. Conclusions Our continuation preliminary results demonstrate that actinidin can reduce collagen I levels in PD cells. However, high doses of actinidin cause cell destruction, so we recommend that treatment should not exceed 20 mg/ml. Future studies will investigate the molecular mechanism by which actinidin hydrolyzes collagen and will evaluate its effects in an animal model. Given the lack of durable FDA-approved treatments for PD in Canada, this novel approach holds significant potential for future treatment options. Disclosure Yes, this is sponsored by industry/sponsor: KiwiEnzyme.com Ltd. Clarification: No industry support in study design or execution.
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Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,001 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,003 | 0,001 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».